دورية أكاديمية
Bisphosphonates attenuate age‐related muscle decline in Caenorhabditis elegans
| العنوان: | Bisphosphonates attenuate age‐related muscle decline in Caenorhabditis elegans |
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| المؤلفون: | Luke Slade, Shelby E. Bollen, Joseph J. Bass, Bethan E. Phillips, Kenneth Smith, Daniel J. Wilkinson, Nathaniel J. Szewczyk, Philip J. Atherton, Timothy Etheridge |
| المصدر: | Journal of Cachexia, Sarcopenia and Muscle, Vol 14, Iss 6, Pp 2613-2622 (2023) |
| بيانات النشر: | Wiley, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Diseases of the musculoskeletal system LCC:Human anatomy |
| مصطلحات موضوعية: | Healthspan, Lifespan, Muscle, Sarcopenia, Zoledronic acid, Diseases of the musculoskeletal system, RC925-935, Human anatomy, QM1-695 |
| الوصف: | Abstract Background Age‐related muscle decline (sarcopenia) associates with numerous health risk factors and poor quality of life. Drugs that counter sarcopenia without harmful side effects are lacking, and repurposing existing pharmaceuticals could expedite realistic clinical options. Recent studies suggest bisphosphonates promote muscle health; however, the efficacy of bisphosphonates as an anti‐sarcopenic therapy is currently unclear. Methods Using Caenorhabditis elegans as a sarcopenia model, we treated animals with 100 nM, 1, 10, 100 and 500 μM zoledronic acid (ZA) and assessed lifespan and healthspan (movement rates) using a microfluidic chip device. The effects of ZA on sarcopenia were examined using GFP‐tagged myofibres or mitochondria at days 0, 4 and 6 post‐adulthood. Mechanisms of ZA‐mediated healthspan extension were determined using combined ZA and targeted RNAi gene knockdown across the life‐course. Results We found 100 nM and 1 μM ZA increased lifespan (P 0.05), whereas 100 and 500 μM ZA were larval lethal. ZA (1 μM) significantly improved myofibrillar structure on days 4 and 6 post‐adulthood (83 and 71% well‐organized myofibres, respectively, vs. 56 and 34% controls, P 0.05). Life/healthspan was extended through knockdown of igdb‐1/FNDC5 (635 ± 10 vs. 523 ± 10% population activity AUC in gene knockdown vs. untreated controls, P 0.05]. Conversely, let‐756/FGF21 and sir‐2.2/SIRT‐4 were dispensable for ZA‐induced healthspan [630 ± 6 vs. 523 ± 10% population activity AUC in knockdown + 1 μM ZA vs. untreated controls, P |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2190-6009 2190-5991 |
| Relation: | https://doaj.org/toc/2190-5991; https://doaj.org/toc/2190-6009 |
| DOI: | 10.1002/jcsm.13335 |
| URL الوصول: | https://doaj.org/article/6bd10457f3044741bde9ad6c45e62c4f |
| رقم الأكسشن: | edsdoj.6bd10457f3044741bde9ad6c45e62c4f |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 21906009 21905991 |
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| DOI: | 10.1002/jcsm.13335 |