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Identification and Analysis of Differentially-Expressed microRNAs in Japanese Encephalitis Virus-Infected PK-15 Cells with Deep Sequencing

التفاصيل البيبلوغرافية
العنوان: Identification and Analysis of Differentially-Expressed microRNAs in Japanese Encephalitis Virus-Infected PK-15 Cells with Deep Sequencing
المؤلفون: Yuhan Cai, Ling Zhu, Yuanchen Zhou, Xiao Liu, Xiaowan Liu, Xinqiong Li, Qiaoli Lang, Xiaogai Qiao, Zhiwen Xu
المصدر: International Journal of Molecular Sciences, Vol 16, Iss 1, Pp 2204-2219 (2015)
بيانات النشر: MDPI AG, 2015.
سنة النشر: 2015
المجموعة: LCC:Biology (General)
LCC:Chemistry
مصطلحات موضوعية: Japanese encephalitis virus, microRNA, pathogenesis, deep sequencing, Biology (General), QH301-705.5, Chemistry, QD1-999
الوصف: Japanese encephalitis virus (JEV), a mosquito-borne Flavivirus, causes acute viral encephalitis with high morbidity and mortality in humans and animals. MicroRNAs (miRNAs) are small noncoding RNAs that are important modulators of the intricate host-pathogen interaction networks. However, our knowledge of the changes that occur in miRNAs in host cells after JEV infection is still limited. To understand the molecular pathogenesis of JEV at the level of posttranscriptional regulation, we used Illumina deep sequencing to sequence two small RNA libraries prepared from PK-15 cells before and after JEV infection. We identified 522 and 427 miRNAs in the infected and uninfected cells, respectively. Overall, 132 miRNAs were expressed significantly differently after challenge with JEV: 78 were upregulated and 54 downregulated. The sequencing results for selected miRNAs were confirmed with RT-qPCR. GO analysis of the host target genes revealed that these dysregulated miRNAs are involved in complex cellular pathways, including the metabolic pathway, inflammatory response and immune response. To our knowledge, this is the first report of the comparative expression of miRNAs in PK-15 cells after JEV infection. Our findings will underpin further studies of miRNAs’ roles in JEV replication and identify potential candidates for antiviral therapies against JEV.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1422-0067
Relation: http://www.mdpi.com/1422-0067/16/1/2204; https://doaj.org/toc/1422-0067
DOI: 10.3390/ijms16012204
URL الوصول: https://doaj.org/article/6be91388bd204dc08add3979ebeb78bd
رقم الأكسشن: edsdoj.6be91388bd204dc08add3979ebeb78bd
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:14220067
DOI:10.3390/ijms16012204