دورية أكاديمية
Pain relief and cartilage repair by Nanofat against osteoarthritis: preclinical and clinical evidence
| العنوان: | Pain relief and cartilage repair by Nanofat against osteoarthritis: preclinical and clinical evidence |
|---|---|
| المؤلفون: | Zuxiang Chen, Yanzhi Ge, Li Zhou, Ting Li, Bo Yan, Junjie Chen, Jiefeng Huang, Wenxi Du, Shuaijie Lv, Peijian Tong, Letian Shan |
| المصدر: | Stem Cell Research & Therapy, Vol 12, Iss 1, Pp 1-15 (2021) |
| بيانات النشر: | BMC, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Medicine (General) LCC:Biochemistry |
| مصطلحات موضوعية: | Nanofat, Adipose tissue-derived stem cells, Osteoarthritis, Paracrine, Conditioned medium, Medicine (General), R5-920, Biochemistry, QD415-436 |
| الوصف: | Abstract Background Osteoarthritis (OA) is the most common joint degenerative disorder, with little effective therapy to date. Nanofat is a cocktail of cells obtained from fat tissue, which possesses regenerative capacity and has a potential in treating OA. This study aimed to determine the anti-OA efficacy of Nanofat from basic and clinical aspects and explore its action mode. Methods Flow cytometry was performed to characterize Nanofat. A monoiodoacetate-induced OA rat model was employed for in vivo study. Cell viability and wound healing assays were conducted for in vitro study. Real-time PCR and Western blot assays were applied to explore the molecular action mode of Nanofat. Moreover, a retrospective analysis was conducted to determine the clinical efficacy and safety of Nanofat on knee OA patients. Results The in vivo results showed that Nanofat significantly attenuated pain symptoms and protected cartilage ECM (Col2) from damage, and its effects were not significantly differed with adipose tissue-derived stem cells (both P > 0.05). The in vitro results showed that Nanofat promoted the cell viability and migration of chondrocytes and significantly restored the IL-1β-induced abnormal gene expressions of Col2, Aggrecan, Sox9, Adamts5, Mmp3, Mmp9 Mmp13, IL-6 and Col10 and protein expressions of Col2, MMP9, MMP13, and Sox9 of chondrocytes. The regulatory actions of Nanofat on these anabolic, catabolic, and hypertrophic molecules of chondrocytes were similar between two treatment routes: co-culture and conditioned medium, suggesting a paracrine-based mode of action of Nanofat. Moreover, the clinical data showed that Nanofat relieved pain and repaired damaged cartilage of OA patients, with no adverse events. Conclusion In sum, this study demonstrated the anti-OA efficacy as well as a paracrine-based action mode of Nanofat, providing novel knowledge of Nanofat and suggesting it as a promising and practical cell therapy for clinical treatment of OA. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1757-6512 |
| Relation: | https://doaj.org/toc/1757-6512 |
| DOI: | 10.1186/s13287-021-02538-9 |
| URL الوصول: | https://doaj.org/article/6c80651d3a2b4c489a39d29a4c615a70 |
| رقم الأكسشن: | edsdoj.6c80651d3a2b4c489a39d29a4c615a70 |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 17576512 |
|---|---|
| DOI: | 10.1186/s13287-021-02538-9 |