دورية أكاديمية
Assaying Effector Cell-to-Cell Mobility in Plant Tissues Identifies Hypermobility and Indirect Manipulation of Plasmodesmata
| العنوان: | Assaying Effector Cell-to-Cell Mobility in Plant Tissues Identifies Hypermobility and Indirect Manipulation of Plasmodesmata |
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| المؤلفون: | Mina Ohtsu, Joanna Jennings, Matthew Johnston, Andrew Breakspear, Xiaokun Liu, Kara Stark, Richard J. Morris, Jeroen de Keijzer, Christine Faulkner |
| المصدر: | Molecular Plant-Microbe Interactions, Vol 37, Iss 2, Pp 84-92 (2024) |
| بيانات النشر: | The American Phytopathological Society, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Microbiology LCC:Botany |
| مصطلحات موضوعية: | fungal effectors, fungus–plant interactions, microscopy and imaging, plasmodesmata, Microbiology, QR1-502, Botany, QK1-989 |
| الوصف: | In plants, plasmodesmata establish cytoplasmic continuity between cells to allow for communication and resource exchange across the cell wall. While plant pathogens use plasmodesmata as a pathway for both molecular and physical invasion, the benefits of molecular invasion (cell-to-cell movement of pathogen effectors) are poorly understood. To establish a methodology for identification and characterization of the cell-to-cell mobility of effectors, we performed a quantitative live imaging-based screen of candidate effectors of the fungal pathogen Colletotrichum higginsianum. We predicted C. higginsianum effectors by their expression profiles, the presence of a secretion signal, and their predicted and in planta localization when fused to green fluorescent protein. We assayed for cell-to-cell mobility of nucleocytosolic effectors and identified 14 that are cell-to-cell mobile. We identified that three of these effectors are “hypermobile,” showing cell-to-cell mobility greater than expected for a protein of that size. To explore the mechanism of hypermobility, we chose two hypermobile effectors and measured their impact on plasmodesmata function and found that even though they show no direct association with plasmodesmata, each increases the transport capacity of plasmodesmata. Thus, our methods for quantitative analysis of cell-to-cell mobility of candidate microbe-derived effectors, or any suite of host proteins, can identify cell-to-cell hypermobility and offer greater understanding of how proteins affect plasmodesmal function and intercellular connectivity. [Graphic: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY 4.0 International license. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1943-7706 0894-0282 |
| Relation: | https://doaj.org/toc/0894-0282; https://doaj.org/toc/1943-7706 |
| DOI: | 10.1094/MPMI-05-23-0052-TA |
| URL الوصول: | https://doaj.org/article/6d9baba16de54d70bf156e27198032cf |
| رقم الأكسشن: | edsdoj.6d9baba16de54d70bf156e27198032cf |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19437706 08940282 |
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| DOI: | 10.1094/MPMI-05-23-0052-TA |