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Functional Analysis of TRPA1, TRPM3, and TRPV1 Channels in Human Dermal Arteries and Their Role in Vascular Modulation

التفاصيل البيبلوغرافية
العنوان: Functional Analysis of TRPA1, TRPM3, and TRPV1 Channels in Human Dermal Arteries and Their Role in Vascular Modulation
المؤلفون: Eduardo Rivera-Mancilla, Linda Al-Hassany, Heleen Marynissen, Dorien Bamps, Ingrid M. Garrelds, Jérôme Cornette, A. H. Jan Danser, Carlos M. Villalón, Jan N. de Hoon, Antoinette MaassenVanDenBrink
المصدر: Pharmaceuticals, Vol 17, Iss 2, p 156 (2024)
بيانات النشر: MDPI AG, 2024.
سنة النشر: 2024
المجموعة: LCC:Medicine
LCC:Pharmacy and materia medica
مصطلحات موضوعية: CGRP, human dermal artery, TRP channels, pharmacological mechanisms, vascular tone, vasodilation, Medicine, Pharmacy and materia medica, RS1-441
الوصف: Transient receptor potential (TRP) channels are pivotal in modulating vascular functions. In fact, topical application of cinnamaldehyde or capsaicin (TRPA1 and TRPV1 channel agonists, respectively) induces “local” changes in blood flow by releasing vasodilator neuropeptides. We investigated TRP channels’ contributions and the pharmacological mechanisms driving vasodilation in human isolated dermal arteries. Ex vivo studies assessed the vascular function of artery segments and analyzed the effects of different compounds. Concentration–response curves to cinnamaldehyde, pregnenolone sulfate (PregS, TRPM3 agonist), and capsaicin were constructed to evaluate the effect of the antagonists HC030031 (TRPA1); isosakuranetin (TRPM3); and capsazepine (TRPV1). Additionally, the antagonists/inhibitors olcegepant (CGRP receptor); L-NAME (nitric oxide synthase); indomethacin (cyclooxygenase); TRAM-34 plus apamin (K+ channels); and MK-801 (NMDA receptors, only for PregS) were used. Moreover, CGRP release was assessed in the organ bath fluid post-agonist-exposure. In dermal arteries, cinnamaldehyde- and capsaicin-induced relaxation remained unchanged after the aforementioned antagonists, while PregS-induced relaxation was significantly inhibited by isosakuranetin, L-NAME and MK-801. Furthermore, there was a significant increase in CGRP levels post-agonist-exposure. In our experimental model, TRPA1 and TRPV1 channels seem not to be involved in cinnamaldehyde- or capsaicin-induced relaxation, respectively, whereas TRPM3 channels contribute to PregS-induced relaxation, possibly via CGRP-independent mechanisms.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1424-8247
Relation: https://www.mdpi.com/1424-8247/17/2/156; https://doaj.org/toc/1424-8247
DOI: 10.3390/ph17020156
URL الوصول: https://doaj.org/article/6da861e0a7624ecc9eac267bb8c3cd87
رقم الأكسشن: edsdoj.6da861e0a7624ecc9eac267bb8c3cd87
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:14248247
DOI:10.3390/ph17020156