دورية أكاديمية

Effects of Hair dyes on Sequence Analysis of Hair Mitochondrial DNA Hypervariable Region 1

التفاصيل البيبلوغرافية
العنوان: Effects of Hair dyes on Sequence Analysis of Hair Mitochondrial DNA Hypervariable Region 1
المؤلفون: halah Al-summarraie, Mohammed Al-Zubaidi, Dhuha Salim نعمة, Sura Nabeel Hameed, Thooalnoon Younes Saleh, haider al-rubai, Dheaa Sh. Zageer, ala bader
المصدر: مجلة مركز بحوث التقنيات الاحيائية, Vol 11, Iss 1 (2017)
بيانات النشر: Al-Nahrain University/ Biotechnology Research Center, 2017.
سنة النشر: 2017
المجموعة: LCC:Biotechnology
LCC:Biology (General)
مصطلحات موضوعية: Biological hair sample, Mitochondrial DNA, human identification, degraded samples, Biotechnology, TP248.13-248.65, Biology (General), QH301-705.5
الوصف: Hair can be a valuable source of DNA especially in forensic casework and for noninvasive studies of human, when blood samples not available. This study emphasizes the impact of hair dyes on DNA sequence analysis. Samples collected from forty Iraqi families; each sample was divided in to two parts hair follicles and hair shaft. DNA extracted by using two different techniques, Phenol-chloroform (organic) method and prepFiler forensic DNA extraction kit. After quantification of DNA by real time PCR to confirm the exact DNA yield, Mitochondrial DNA (MtDNA) hypervariable region 1 successfully amplified from (50% of samples include hair follicle and 20% samples include shaft only), which all extracted by organic method. Whereas by using prepFiler kit the ratio of amplification success reach to 95% of samples included hair follicles, but there wais no DNA outcome from hair shaft by using this kit. Our results demonstrate that treated hair by dyed or henna had a significant influence on the sequence analysis results. Organic method was an appropriate method for extraction DNA from hair shaft, since this method used for extracting the old and degraded samples. While prepFiler DNA extraction kit was more convenient for isolation DNA from, hair samples included follicles only with excellent result.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1815-1140
2708-1370
Relation: https://www.jobrc.org/index.php/jobrc/article/view/505; https://doaj.org/toc/1815-1140; https://doaj.org/toc/2708-1370
DOI: 10.24126/jobrc.2017.11.1.505
URL الوصول: https://doaj.org/article/6de709a7efc546d087a7c6564b30f030
رقم الأكسشن: edsdoj.6de709a7efc546d087a7c6564b30f030
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:18151140
27081370
DOI:10.24126/jobrc.2017.11.1.505