دورية أكاديمية
Enzymatic synthesis of indigo derivatives by tuning P450 BM3 peroxygenases
| العنوان: | Enzymatic synthesis of indigo derivatives by tuning P450 BM3 peroxygenases |
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| المؤلفون: | Li Ma, Tianjian Sun, Yunjie Liu, Yue Zhao, Xiaohui Liu, Yuxuan Li, Xinwei Chen, Lin Cao, Qianqian Kang, Jiawei Guo, Lei Du, Wei Wang, Shengying Li |
| المصدر: | Synthetic and Systems Biotechnology, Vol 8, Iss 3, Pp 452-461 (2023) |
| بيانات النشر: | KeAi Communications Co., Ltd., 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biotechnology LCC:Biology (General) |
| مصطلحات موضوعية: | Cytochrome P450 enzymes, P450 BM3 peroxygenase, Indigoids, Dyestuff, Organic electronics, Biotechnology, TP248.13-248.65, Biology (General), QH301-705.5 |
| الوصف: | Indigoids, a class of bis-indoles, have long been applied in dyeing, food, and pharmaceutical industries. Recently, interest in these ‘old’ molecules has been renewed in the field of organic semiconductors as functional building blocks for organic electronics due to their excellent chemical and physical properties. However, these indigo derivatives are difficult to access through chemical synthesis. In this study, we engineer cytochrome P450 BM3 from an NADPH-dependent monooxygenase to peroxygenases through directed evolution. A select number of P450 BM3 variants are used for the selective oxidation of indole derivatives to form different indigoid pigments with a spectrum of colors. Among the prepared indigoid organic photocatalysts, a majority of indigoids demonstrate a reduced band gap than indigo due to the increased light capture and improved charge separation, making them promising candidates for the development of new organic electronic devices. Thus, we present a useful enzymatic approach with broad substrate scope and cost-effectiveness by using low-cost H2O2 as a cofactor for the preparation of diversified indigoids, offering versatility in designing and manufacturing new dyestuff and electronic/sensor components. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2405-805X |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2405805X23000510; https://doaj.org/toc/2405-805X |
| DOI: | 10.1016/j.synbio.2023.06.006 |
| URL الوصول: | https://doaj.org/article/6e1a5288ce6a4117a53aee393ffc910e |
| رقم الأكسشن: | edsdoj.6e1a5288ce6a4117a53aee393ffc910e |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2405805X |
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| DOI: | 10.1016/j.synbio.2023.06.006 |