Effects of injecting L-Arginine on immune responses and intestinal structure of the hybrid grouper (Epinephelus fuscoguttatus ♀× Epinephelus lanceolatus ♂)
التفاصيل البيبلوغرافية
العنوان:
Effects of injecting L-Arginine on immune responses and intestinal structure of the hybrid grouper (Epinephelus fuscoguttatus ♀× Epinephelus lanceolatus ♂)
To study the effects of L-Arginine (Arg) on immune responses and intestinal structure in a hybrid grouper (Epinephelus fuscoguttatus ♀× Epinephelus lanceolatus ♂), intraperitoneal injection of different Arg doses (0, 50, 100, 200 mg per kg body weight of grouper) was administered once every seven days, repeating the process three times in the present study. A total of 216 groupers (initial weight of 349.42 ± 0.67 g) were randomly divided into four groups with three replicates, and each of the replicates comprised 18 fish. Serum was sampled at the seven, 14, and 21 days post-injection. The results showed that injection of Arg had a significant effect on serum albumin, immunoglobulin M (IgM), total protein (TP) and nitric oxide (NO) content, as well as nitric oxide synthase (NOS) and lysozyme activity (P
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