دورية أكاديمية
Different patterns of collagen-proteoglycan interaction: a scanning electron microscopyand atomic force microscopy study
| العنوان: | Different patterns of collagen-proteoglycan interaction: a scanning electron microscopyand atomic force microscopy study |
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| المؤلفون: | A Ruggeri, P Gobbi, A Alessandrini, T Congiu, M Raspanti |
| المصدر: | European Journal of Histochemistry, Vol 44, Iss 4, Pp 335-43 (2009) |
| بيانات النشر: | PAGEPress Publications, 2009. |
| سنة النشر: | 2009 |
| المجموعة: | LCC:Biology (General) |
| مصطلحات موضوعية: | Biology (General), QH301-705.5 |
| الوصف: | The extracellular matrix of unfixed, unstained rat corneal stroma, visualized with high-resolution scanning electron microscopy and atomic force microscopy after minimal preliminary treatment, appears composed of straight, parallel, uniform collagen fibrils regularly spaced by a three-dimensional, irregular network of thin, delicate proteoglycan filaments. Rat tail tendon, observed under identical conditions, appears instead made of heterogeneous, closely packed fibrils interwoven with orthogonal proteoglycan filaments. Pre-treatment with cupromeronic blue just thickens the filaments without affecting their spatial layout. Digestion with chondroitinase ABC rids the tendon matrix of all its interconnecting filaments while the corneal stroma architecture remains virtually unaffected, its fibrils always being separated by an evident interfibrillar spacing which is never observed in tendon. Our observations indicate that matrix proteoglycans are responsible for both the highly regular interfibrillar spacing which is distinctive of corneal stroma, and the strong interfibrillar binding observed in tendon. These opposite interaction patterns appear to be distinctive of different proteo- glycan species. The molecular details of proteoglycan interactions are still incompletely understood and are the subject of ongoing research. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1121-760X 2038-8306 |
| Relation: | http://ejh.pagepress.org/index.php/ejh/article/view/1596; https://doaj.org/toc/1121-760X; https://doaj.org/toc/2038-8306 |
| DOI: | 10.4081/1596 |
| URL الوصول: | https://doaj.org/article/6e9fcc00286d42cba8ffd7890bd16b08 |
| رقم الأكسشن: | edsdoj.6e9fcc00286d42cba8ffd7890bd16b08 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 1121760X 20388306 |
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| DOI: | 10.4081/1596 |