دورية أكاديمية
In Vitro Comparative Study of Near-Infrared Photoimmunotherapy and Photodynamic Therapy
| العنوان: | In Vitro Comparative Study of Near-Infrared Photoimmunotherapy and Photodynamic Therapy |
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| المؤلفون: | Susumu Yamashita, Miho Kojima, Nobuhiko Onda, Makoto Shibutani |
| المصدر: | Cancers, Vol 15, Iss 13, p 3400 (2023) |
| بيانات النشر: | MDPI AG, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens |
| مصطلحات موضوعية: | near-infrared photoimmunotherapy (NIR-PIT), monoclonal antibody (mAb), IRDye700, photodynamic therapy (PDT), talaporfin sodium, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282 |
| الوصف: | Near-infrared photoimmunotherapy (NIR-PIT) is a new phototherapy that utilizes a monoclonal antibody (mAb) against cancer antigens and a phthalocyanine dye, IRDye700DX (IR700) conjugate (mAb-IR700). Photodynamic therapy (PDT) is a combination therapy that utilizes photoreactive agents and light irradiation as well as NIR-PIT. In the present study, we compared these therapies in vitro. The characterization of cellular binding/uptake specificity and cytotoxicity were examined using two mAb-IR700 forms and a conventional PDT agent, talaporfin sodium, in three cell lines. As designed, mAb-IR700 had high molecular selectivity and visualized target molecule-positive cells at the lowest concentration examined. NIR-PIT induced necrosis and damage-associated molecular patterns (DAMPs), a surrogate maker of immunogenic cell death. In contrast, talaporfin sodium was taken up by cells regardless of cell type, and its uptake was enhanced in a concentration-dependent manner. PDT induced cell death, with the pattern of cell death shifting from apoptosis to necrosis depending on the concentration of the photosensitizer. Induction of DAMPs was observed at the highest concentration, but their sensitivity differed among cell lines. Overall, our data suggest that molecule-specific NIR-PIT may have potential advantages compared with PDT in terms of the efficiency of tumor visualization and induction of DAMPs. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 15133400 2072-6694 |
| Relation: | https://www.mdpi.com/2072-6694/15/13/3400; https://doaj.org/toc/2072-6694 |
| DOI: | 10.3390/cancers15133400 |
| URL الوصول: | https://doaj.org/article/6ea37fcaf18d41f38a76188ab428578a |
| رقم الأكسشن: | edsdoj.6ea37fcaf18d41f38a76188ab428578a |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 15133400 20726694 |
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| DOI: | 10.3390/cancers15133400 |