دورية أكاديمية
Leishmaniasis in Turkey: Determination of Leishmania Species by Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS)
| العنوان: | Leishmaniasis in Turkey: Determination of Leishmania Species by Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) |
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| المؤلفون: | Gülnaz Culha, Isin Akyar, Fadile Yildiz Zeyrek, Özgür Kurt, Cumhur Gündüz, Seray Özensoy Töz, Ipek Östan, Ibrahim Cavus, Burcu Gülkan, Tanil Kocagöz, Yusuf Özbel, Ahmet Özbilgin |
| المصدر: | Iranian Journal of Parasitology, Vol 9, Iss 2 (2014) |
| بيانات النشر: | Tehran University of Medical Sciences, 2014. |
| سنة النشر: | 2014 |
| المجموعة: | LCC:Infectious and parasitic diseases |
| مصطلحات موضوعية: | Cutaneous leishmaniasis, Leishmania infantum, MALDI -TOF, Real-Time PCR, Turkey, Infectious and parasitic diseases, RC109-216 |
| الوصف: | Background: Cutaneous leishmaniasis (CL) is endemic in Southeastern Anatolia, mainly in Sanliurfa and Hatay provinces, and the causative agents are mostly Leishmania tropica and less frequently L. infantum. Here, we report the first MAL-DI-TOF analyses of Leishmania promastigotes obtained from the cultures of two CL cases from Osmaniye and Hatay provinces who were initially diagnosed by microscopy, culture and identified as L. infantum with Real-Time PCR (RT-PCR). Methods: Samples obtained from the skin lesions of patients were initially stained with Giemsa and cultivated in NNN medium. Examination of the smears and cultures revealed Leishmania amastigotes and promastigotes, respectively. The promastigotes (MHOM/TR/2012/CBU15 and MHOM/TR/2012/MK05) ob-tained from the cultures of both patients were used for RT-PCR targeting the ITS-1 region in the SSU of rRNA. The reference strains of four Leishmania spe-cies (L. infantum, L. donovani, L. tropica and L. major) were initially assessed with MALDI-TOF and their data were added to MALDI-TOF Biotyper Library. Results: Both RT-PCR and MALDI-TOF analyses indicated that the causative agent in both patient samples was L. infantum. Conclusion: Despite disadvantages such as requirement of culture fluid with nothing but promastigotes and high cost, MALDI-TOF analysis may be a fast, sensitive and specific diagnostic tool in especially large-scale research studies, where the cost declines, relatively. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1735-7020 2008-238X |
| Relation: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/407; https://doaj.org/toc/1735-7020; https://doaj.org/toc/2008-238X |
| URL الوصول: | https://doaj.org/article/6f0e9e2079b3461d9a7a3f3348726954 |
| رقم الأكسشن: | edsdoj.6f0e9e2079b3461d9a7a3f3348726954 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 17357020 2008238X |
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