دورية أكاديمية
Improving precision of proximity ligation assay by amplified single molecule detection.
| العنوان: | Improving precision of proximity ligation assay by amplified single molecule detection. |
|---|---|
| المؤلفون: | Rongqin Ke, Rachel Yuan Nong, Simon Fredriksson, Ulf Landegren, Mats Nilsson |
| المصدر: | PLoS ONE, Vol 8, Iss 7, p e69813 (2013) |
| بيانات النشر: | Public Library of Science (PLoS), 2013. |
| سنة النشر: | 2013 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | Proximity ligation assay (PLA) has been proven to be a robust protein detection method. The technique is characterized by high sensitivity and specificity, but the assay precision is probably limited by the PCR readout. To investigate this potential limitation and to improve precision, we developed a digital proximity ligation assay for protein measurement in fluids based on amplified single molecule detection. The assay showed significant improvements in precision, and thereby also detection sensitivity, over the conventional real-time PCR readout. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1932-6203 |
| Relation: | http://europepmc.org/articles/PMC3713053?pdf=render; https://doaj.org/toc/1932-6203 |
| DOI: | 10.1371/journal.pone.0069813 |
| URL الوصول: | https://doaj.org/article/6f9414c9ca614aa098ffc687babfe575 |
| رقم الأكسشن: | edsdoj.6f9414c9ca614aa098ffc687babfe575 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19326203 |
|---|---|
| DOI: | 10.1371/journal.pone.0069813 |