دورية أكاديمية
Association of HHV-6 with Hodgkin and non Hodgkin lymphoma
| العنوان: | Association of HHV-6 with Hodgkin and non Hodgkin lymphoma |
|---|---|
| المؤلفون: | Hadis Kiani, Manoochehr Makvandi, Alireza Samarbafzadeh, Ali Teimoori, Niloofar Neisi, Hamideh Mehravaran, Hashem Radmehr, Zeinab Hosseini, Azadeh Haghi, Toran Shahani, Mehran Varnaseri, Nastaran Ranjbari |
| المصدر: | Iranian Journal of Microbiology, Vol 8, Iss 2 (2016) |
| بيانات النشر: | Tehran University of Medical Sciences, 2016. |
| سنة النشر: | 2016 |
| المجموعة: | LCC:Microbiology |
| مصطلحات موضوعية: | Nested PCR, Human Herpes virus 6, Hodgkin lymphoma, Non-Hodgkin lymphoma, Microbiology, QR1-502 |
| الوصف: | Background and Objectives: Human Herpes 6 virus (HHV-6) could remain latent and chronic in the host cells after primary infection. HHV-6 genome encodes certain trans activation proteins which may results in development of malignantlymphoma. The association of human herpes six virus (HHV-6) infection and Hodgkin and Non-Hodgkin lymphomas is strongly supported by epidemiological studies. The aim of this study was to determine the prevalence of HHV-6 among the patients with Hodgkin, Non- Hodgkin`s lymphoma. Materials and Methods: Overall 44 blocks of formalin-fixed, paraffin-embedded of the patients including 22(50%) Hodgkin and 22(50%) Non-Hodgkin Lymphoma were collected. Initially the section of 5μm-thickness were prepared from the formalin-fixed, paraffin-embedded tissue blocks. Then the deparaphinazation was carried out for each sample. The DNA was extracted, followed by nested PCR for detection of HHV-6. Based on PCR product size and sequencing, the HHV-6 A or B subtypes were characterized. Results: 12/22(54.54%) cases of Hodgkin and 8/22 (36.36%) Non-Hodgkin’s lymphoma were shown as positive for HHV-6. Out of 12 positive HHV-6 in Hodgkin lymphoma, 10 patients (45.45%) belonged to variant A while 2 cases (9.09%) were found positive for both HHV-6A and HHV-6B. All the Non Hodgkin samples (n=8, 36.36%) showed positive for HHV-6 variant A. Conclusion: High prevalence of HHV-6 was found among the patients with Hodgkin and Non-Hodgkin’s lymphoma. Two patients with Hodgkin lymphoma had mixed HHV-6A and HHV-6B infections. It is recommended patients with Hodgkin and Non-Hodgkin should be screened for HHV-6 detection before chemotherapy. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2008-3289 2008-4447 |
| Relation: | https://ijm.tums.ac.ir/index.php/ijm/article/view/833; https://doaj.org/toc/2008-3289; https://doaj.org/toc/2008-4447 |
| URL الوصول: | https://doaj.org/article/705ecadb64bc44859c07483bf533c3d8 |
| رقم الأكسشن: | edsdoj.705ecadb64bc44859c07483bf533c3d8 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20083289 20084447 |
|---|