دورية أكاديمية
Biallelic modification of IL2RG leads to severe combined immunodeficiency in pigs
| العنوان: | Biallelic modification of IL2RG leads to severe combined immunodeficiency in pigs |
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| المؤلفون: | Jung-Taek Kang, Bumrae Cho, Junghyun Ryu, Caitlin Ray, Eun-Jin Lee, Yun-Jin Yun, SunMi Ahn, JinSeok Lee, Dal-Young Ji, Nathaniel Jue, Sherrie Clark-Deener, Kiho Lee, Kwang-Wook Park |
| المصدر: | Reproductive Biology and Endocrinology, Vol 14, Iss 1, Pp 1-9 (2016) |
| بيانات النشر: | BMC, 2016. |
| سنة النشر: | 2016 |
| المجموعة: | LCC:Gynecology and obstetrics LCC:Reproduction |
| مصطلحات موضوعية: | IL2RG, Severe Combined Immunodeficiency (SCID), CRISPR/Cas9, Somatic cell nuclear transfer (SCNT), Knockout pigs, Gynecology and obstetrics, RG1-991, Reproduction, QH471-489 |
| الوصف: | Abstracts Background Pigs with SCID can be a useful model in regenerative medicine, xenotransplantation, and cancer cell transplantation studies. Utilizing genome editing technologies such as CRISPR/Cas9 system allows us to generate genetically engineered pigs at a higher efficiency. In this study, we report generation and phenotypic characterization of IL2RG knockout female pigs produced through combination of CRISPR/Cas9 system and SCNT. As expected, pigs lacking IL2RG presented SCID phenotype. Methods First, specific CRISPR/Cas9 systems targeting IL2RG were introduced into developing pig embryos then the embryos were transferred into surrogates. A total of six fetuses were obtained from the embryo transfer and fetal fibroblast cell lines were established. Then IL2RG knockout female cells carrying biallelic genetic modification were used as donor cells for SCNT, followed by embryo transfer. Results Three live cloned female piglets carrying biallelic mutations in IL2RG were produced. All cloned piglets completely lacked thymus and they had a significantly reduced level of mature T, B and NK cells in their blood and spleen. Conclusions Here, we generated IL2RG knockout female pigs showing phenotypic characterization of SCID. This IL2RG knockout female pigs will be a promising model for biomedical and translational research. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1477-7827 |
| Relation: | http://link.springer.com/article/10.1186/s12958-016-0206-5; https://doaj.org/toc/1477-7827 |
| DOI: | 10.1186/s12958-016-0206-5 |
| URL الوصول: | https://doaj.org/article/c706b51900514cef8e5f11e7865aecf2 |
| رقم الأكسشن: | edsdoj.706b51900514cef8e5f11e7865aecf2 |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 14777827 |
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| DOI: | 10.1186/s12958-016-0206-5 |