دورية أكاديمية
The molecular characterization of colistin-resistant isolates of Acinetobacter baumannii from patients at intensive care units
العنوان: | The molecular characterization of colistin-resistant isolates of Acinetobacter baumannii from patients at intensive care units |
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المؤلفون: | Safar Farajnia, Fariba Lotfi, Alireza Dehnad, Maryam Shojaie, Roya Raisi, Leila Rahbarnia, Ahad Bazmani, Behrooz Naghili, Samaneh Shiry |
المصدر: | Iranian Journal of Microbiology, Vol 14, Iss 3 (2022) |
بيانات النشر: | Tehran University of Medical Sciences, 2022. |
سنة النشر: | 2022 |
المجموعة: | LCC:Microbiology |
مصطلحات موضوعية: | Acinetobacter baumannii, Beta-lactamase OXA-23, Beta-lactamase OXA-143, OXA-72 carbapenemase, Beta-lactamase OXA-58, Colistin, Microbiology, QR1-502 |
الوصف: | Background and Objectives: The objective of this study was to determine molecular characterization and genetic diversity of colistin-resistant A. baumannii clinical isolates in Intensive Care Unit hospitalized patients. Materials and Methods: A total of 127 A. baumannii clinical isolates were evaluated for antimicrobial susceptibility. PCR reaction and sequencing were performed for the detection of mutations in pmrAB and lpx ACD genes. Results: Based on antimicrobial susceptibility testing, 40.94% and 33.85% of the isolates were MDR and XDR respectively whereas 3.93% of them were found to be PDR. Results of agar dilution MIC and E-test indicated that 76% of the isolates were sensitive to colistin. All of the isolates were positive for blaOXA-51 and 50% of them were positive for both blaOXA-23-like and blaOXA-143-like genes while only 25% of the isolates were positive for blaOXA-72. None of them were positive for the blaOXA-58-like gene. There is no mutation in pmrA. The V162A substitution for pmrB gene was repeated in two isolates, and E394 D and Y292H substitutions in lpxA were observed in two isolates; also, C120R and F165L substitutions in lpxC gene was repeated in two isolates. Analysis of phylogenetic tree based on alterations in lpxACD and pmrB genes indicated the appearance of new isolates compared to the reference strain ATCC17978 A. baumannii isolates. Conclusion: The present study indicated the prevalence of MDR and XDR A. baumannii isolates and the emergence of PDR isolates in the northwest portion of Iran. The appearance of colistin-resistant isolates with new mutations in pmrB, lpxACD genes indicates new resistance mechanisms. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 2008-3289 2008-4447 |
Relation: | https://ijm.tums.ac.ir/index.php/ijm/article/view/3400; https://doaj.org/toc/2008-3289; https://doaj.org/toc/2008-4447 |
URL الوصول: | https://doaj.org/article/dec708754d274133a641415523cd3b7e |
رقم الأكسشن: | edsdoj.708754d274133a641415523cd3b7e |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 20083289 20084447 |
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