دورية أكاديمية

Up-regulation of MARVEL domain-containing protein 1 (MARVELD1) accelerated the malignant phenotype of glioma cancer cells via mediating JAK/STAT signaling pathway

التفاصيل البيبلوغرافية
العنوان: Up-regulation of MARVEL domain-containing protein 1 (MARVELD1) accelerated the malignant phenotype of glioma cancer cells via mediating JAK/STAT signaling pathway
المؤلفون: Lingyang Xia, Peng Jin, Wei Tian, Shuang Liang, Liye Tan, Binxin Li
المصدر: Brazilian Journal of Medical and Biological Research, Vol 54, Iss 7 (2021)
بيانات النشر: Associação Brasileira de Divulgação Científica, 2021.
سنة النشر: 2021
المجموعة: LCC:Medicine (General)
LCC:Biology (General)
مصطلحات موضوعية: MARVELD1, Glioma, Prognosis, Functional analysis, JAK/STAT signaling pathway, Medicine (General), R5-920, Biology (General), QH301-705.5
الوصف: This work aimed to research the function of MARVEL domain-containing protein 1 (MARVELD1) in glioma as well as its functioning mode. Bioinformatics analysis was utilized to assess the MARVELD1 expression in glioma tissues and its relationship with grade and prognosis, based on The Cancer Genome Atlas (TCGA), Genotype-Tissue Expression (GTEx), and Chinese Glioma Genome Atlas (CGGA) databases. Cell Counting Kit-8 (CCK-8), colony formation, and Transwell assays were carried out to determine the impact of MARVELD1 on malignant biological behavior of glioma, such as proliferation, invasion, and migration. qRT-PCR was carried out to test the mRNA level of MARVELD1. Western blot assay was performed to measure the protein expression of MARVELD1 and JAK/STAT pathway-related proteins. MARVELD1 was expressed at high levels in glioma tissues and cell lines. Kaplan-Meier survival analysis revealed that the higher MARVELD1 expression, the shorter the survival time of patients with glioma. Also, the MARVELD1 expression in WHO IV was significantly enhanced compared to that in WHO II and WHO III. Furthermore, the functional analysis of MARVELD1 in vitro revealed that knockdown of MARVELD1 in U251 cells restrained cell proliferation, migration, and invasion, while up-regulation of MARVELD1 in U87 cells presented opposite outcomes. Finally, we found that JAK/STAT signaling pathway mediated the function of MARVELD1 in glioma. MARVELD1 contributed to promoting the malignant progression of glioma, which is the key driver of activation of JAK/STAT signaling pathway in gliomas.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1414-431X
1414-431x
Relation: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021000700606&tlng=en; http://www.scielo.br/pdf/bjmbr/v54n7/1414-431X-bjmbr-54-7-e10236.pdf; https://doaj.org/toc/1414-431X
DOI: 10.1590/1414-431x2020e10236
URL الوصول: https://doaj.org/article/70b7297efd5042ad88502d59b501a9a6
رقم الأكسشن: edsdoj.70b7297efd5042ad88502d59b501a9a6
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:1414431X
1414431x
DOI:10.1590/1414-431x2020e10236