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CHARACTERIZATION OF 18S RIBOSOMAL RNA FRAGMENT FROM Solanum tuberosum L. var. Granola POTATO

التفاصيل البيبلوغرافية
العنوان: CHARACTERIZATION OF 18S RIBOSOMAL RNA FRAGMENT FROM Solanum tuberosum L. var. Granola POTATO
المؤلفون: Yunior William Susanto, Hermin Pancasakti Kusumaningrum, Siti Nur Jannah, Sri Rustini
المصدر: Scripta Biologica, Vol 5, Iss 1 (2018)
بيانات النشر: Jenderal Soedirman University, 2018.
سنة النشر: 2018
المجموعة: LCC:Science
LCC:Biology (General)
مصطلحات موضوعية: Granola, characterization, 18S rRNA, sequencing, phylogenetics, Science, Biology (General), QH301-705.5
الوصف: Potato (Solanum tuberosum L.) is a prime horticultural commodity. One of the varieties of potato that widely cultivated in Indonesia is Granola. This study characterized the variety Granola based on the 18S sequences of rRNA gene fragment. The 18S sequences were used to distinguish the Granola and determine the differentiating characters from other Solanum based on those sequences data. The characterization was completed in three main steps including DNA isolation from potato leaf using Doyle & Doyle method, amplification of the 18S gene fragment, and DNA sequencing. The amplification of 18S gene fragment by a PCR method obtained 528 bp sequences. The BLAST search using NCBI web service confirmed that Granola potato has 99% matching sequence with S. tuberosum. The phylogenetic reconstruction further indicates the S. tuberosum var. Granola used in this study deeply nested with the reference sequence X67238.1, a potato from Europe.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: Indonesian
تدمد: 2355-3138
Relation: https://journal.bio.unsoed.ac.id/index.php/scribio/article/view/760; https://doaj.org/toc/2355-3138
DOI: 10.20884/1.sb.2018.5.1.760
URL الوصول: https://doaj.org/article/70c6b8bee92341868808f66140b30d3d
رقم الأكسشن: edsdoj.70c6b8bee92341868808f66140b30d3d
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:23553138
DOI:10.20884/1.sb.2018.5.1.760