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MEK and TGF-beta Inhibition Promotes Reprogramming without the Use of Transcription Factor.

التفاصيل البيبلوغرافية
العنوان: MEK and TGF-beta Inhibition Promotes Reprogramming without the Use of Transcription Factor.
المؤلفون: Jan Vrbsky, Tamas Tereh, Sergiy Kyrylenko, Petr Dvorak, Lumir Krejci
المصدر: PLoS ONE, Vol 10, Iss 6, p e0127739 (2015)
بيانات النشر: Public Library of Science (PLoS), 2015.
سنة النشر: 2015
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: The possibility of replacing the originally discovered and widely used DNA reprogramming transcription factors is stimulating enormous effort to identify more effective compounds that would not alter the genetic information. Here, we describe the generation of induced pluripotent stem cells (iPSc) from head-derived primary culture of mouse embryonic cells using small chemical inhibitors of the MEK and TGF-beta pathways without delivery of exogenous transcription factors. These iPSc express standard pluripotency markers and retain their potential to differentiate into cells of all germ layers. Our data indicate that head-derived embryonic neural cells might have the reprogramming potential while neither the same primary cells cultivated over five passages in vitro nor a cell population derived from adult brain possesses this capacity. Our results reveal the potential for small molecules to functionally replace routinely used transcription factors and lift the veil on molecular regulation controlling pluripotency. The conditions described here could provide a platform upon which other genome non integrative and safer reprogramming processes could be developed. This work also shows novel potential for developing embryonic neural cells.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1932-6203
Relation: http://europepmc.org/articles/PMC4454598?pdf=render; https://doaj.org/toc/1932-6203
DOI: 10.1371/journal.pone.0127739
URL الوصول: https://doaj.org/article/710c3eab7a05477e843eea3325b8402c
رقم الأكسشن: edsdoj.710c3eab7a05477e843eea3325b8402c
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19326203
DOI:10.1371/journal.pone.0127739