دورية أكاديمية

Mechanism of Anti-Trypanosoma cruzi Action of Gold(I) Compounds: A Theoretical and Experimental Approach

التفاصيل البيبلوغرافية
العنوان: Mechanism of Anti-Trypanosoma cruzi Action of Gold(I) Compounds: A Theoretical and Experimental Approach
المؤلفون: Javiera Órdenes-Rojas, Paola Risco, José Ortega-Campos, Germán Barriga-González, Ana Liempi, Ulrike Kemmerling, Dinorah Gambino, Lucía Otero, Claudio Olea Azar, Esteban Rodríguez-Arce
المصدر: Inorganics, Vol 12, Iss 5, p 133 (2024)
بيانات النشر: MDPI AG, 2024.
سنة النشر: 2024
المجموعة: LCC:Inorganic chemistry
مصطلحات موضوعية: gold compounds, thiosemicarbazones, Trypanosoma cruzi, free radicals, Inorganic chemistry, QD146-197
الوصف: In the search for a more effective chemotherapy for the treatment of Chagas’ disease, caused by Trypanosoma cruzi parasite, the use of gold compounds may be a promising approach. In this work, four gold(I) compounds [AuCl(HL)], (HL = bioactive 5-nitrofuryl containing thiosemicarbazones) were studied. The compounds were theoretically characterized, showing identical chemical structures with the metal ion located in a linear coordination environment and the thiosemicarbazones acting as monodentate ligands. Cyclic voltammetry and Electron Spin Resonance (ESR) studies demonstrated that the complexes could generate the nitro anion radical (NO2−) by reduction of the nitro moiety. The compounds were evaluated in vitro on the trypomastigote form of T. cruzi and human cells of endothelial morphology. The gold compounds studied showed activity in the micromolar range against T. cruzi. The most active compounds (IC50 of around 10 μM) showed an enhancement of the antiparasitic activity compared with their respective bioactive ligands and moderate selectivity. To get insight into the anti-chagasic mechanism of action, the intracellular free radical production capacity of the gold compounds was assessed by ESR and fluorescence measurements. DMPO (5,5-dimethyl-1-pirroline-N-oxide) spin adducts related to the bioreduction of the complexes and redox cycling processes were characterized. The potential oxidative stress mechanism against T. cruzi was confirmed.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2304-6740
Relation: https://www.mdpi.com/2304-6740/12/5/133; https://doaj.org/toc/2304-6740
DOI: 10.3390/inorganics12050133
URL الوصول: https://doaj.org/article/c7171937b9434b69939630d12b86a655
رقم الأكسشن: edsdoj.7171937b9434b69939630d12b86a655
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:23046740
DOI:10.3390/inorganics12050133