دورية أكاديمية
Heterologous Prime-Boost Immunization with DNA Vaccine and Modified Recombinant Proteins Enhances Immune Response against Trueperella pyogenes in Mice
| العنوان: | Heterologous Prime-Boost Immunization with DNA Vaccine and Modified Recombinant Proteins Enhances Immune Response against Trueperella pyogenes in Mice |
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| المؤلفون: | Ting Huang, Kelei Zhao, Xuhao Song, Tao Song, Xinrong Wang, Xiuyue Zhang, Bisong Yue, Yiwen Chu |
| المصدر: | Vaccines, Vol 10, Iss 6, p 839 (2022) |
| بيانات النشر: | MDPI AG, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Medicine |
| مصطلحات موضوعية: | Trueperella pyogenes, DNA vaccine, recombinant proteins, heterologous prime-boost, pyolysin, Medicine |
| الوصف: | Trueperella pyogenes (T. pyogenes) is a crucial opportunistic pathogen normally causing mastitis, abscesses and pneumonia in economically important ruminants. Although only one commercial vaccine of T. pyogenes is currently obtainable, its immunoprotective effect is limited. Pyolysin (PLO) is the most predominant virulence factor highly expressed in T. pyogenes and is an excellent target for the development of novel vaccines against T. pyogenes. In this study, we designed a heterologous prime-boost vaccination scheme combining a DNA vaccine pVAX1-PLO and a subunit vaccine His-PLO to maximize host responses in mice. Humoral and cellular immune responses and protective effects were evaluated in mice to compare the immunogenicity induced by different immunization schemes. Compared to the PBS-control group, in vivo immunization results showed that better immune responses of mice immunized with the pVAX1-PLO plasmids and His-PLO proteins were induced. The residual bacterial burdens from the liver and peritoneal fluid were remarkably decreased in the immunized mice compared with the PBS group. Notably, the heterologous prime-boost vaccination groups significantly enhanced host humoral and cellular immune responses and protected mice from different virulent T. pyogenes strains infection. Conclusively, this study provides a favorable strategy for the further development of next-generation vaccines against T. pyogenes infections. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 10060839 2076-393X |
| Relation: | https://www.mdpi.com/2076-393X/10/6/839; https://doaj.org/toc/2076-393X |
| DOI: | 10.3390/vaccines10060839 |
| URL الوصول: | https://doaj.org/article/e717847ab7cb4810bb38cc5d4e29ec53 |
| رقم الأكسشن: | edsdoj.717847ab7cb4810bb38cc5d4e29ec53 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 10060839 2076393X |
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| DOI: | 10.3390/vaccines10060839 |