دورية أكاديمية
Determination of thiourea in wheat flour and flour treatment agents by pass-through solid phase extraction purification and high performance liquid chromatography
| العنوان: | Determination of thiourea in wheat flour and flour treatment agents by pass-through solid phase extraction purification and high performance liquid chromatography |
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| المؤلفون: | DING Yi, XUE Xia, LU Lanxiang, WEI Lili, CUI Yuhua, CHENG Zhi, CAI Ruiqi, WANG Jun, HU Mei, LIU Yanming |
| المصدر: | Zhongguo shipin weisheng zazhi, Vol 34, Iss 4, Pp 750-754 (2022) |
| بيانات النشر: | The Editorial Office of Chinese Journal of Food Hygiene, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Food processing and manufacture LCC:Nutrition. Foods and food supply |
| مصطلحات موضوعية: | wheat flour, flour treatment agents, thiourea, pass-through solid phase extraction, high performance liquid chromatography, Food processing and manufacture, TP368-456, Nutrition. Foods and food supply, TX341-641 |
| الوصف: | ObjectiveTo establish a detection method for thiourea in wheat flour and flour treatment agents（FTA） by pass-through solid phase extraction purification and high performance liquid chromatography.MethodsThe samples were extracted by acetonitrile aqueous solution（8：2， V/V）， and purified by PRiME HLB pass-through SPE column. The extract was separated on a XBridge Amide column （4.6 mm×250 mm， 5 μm） with acetonitrile and water as the mobile phase in gradient elution， and detected by high performance liquid chromatography-diode array detector （HPLC-DAD）.ResultsThe linear correlation coefficient （R2） was higher than 0. 9999 in the range of 0.05-20 μg/mL. The limit of detection （LOD） and the limit of quantification （LOQ） were 0.15 and 0.5 mg/kg， respectively. The average recoveries ranged from 95.5% to 103.7% with the relative standard deviation （RSD） less than 5.7% at three spiked levels of LOQ， 2LOQ and 10LOQ.ConclusionThe problem of easy gelation and poor dispersion effect of FTA had been solved by the method established in this experiment， while matrix interference in wheat flour and FTA reduced effectively， which protected the chromatographic column. This simple， sensitive， accurate method is suitable for the confirmation and quantification of thiourea in wheat flour and FTA. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | Chinese |
| تدمد: | 1004-8456 |
| Relation: | http://www.zgspws.com/zgspwszz/article/abstract/202204018?st=article_issue; https://doaj.org/toc/1004-8456 |
| DOI: | 10.13590/j.cjfh.2022.04.018 |
| URL الوصول: | https://doaj.org/article/a71d9fdbd49b40a08c346587c622bd51 |
| رقم الأكسشن: | edsdoj.71d9fdbd49b40a08c346587c622bd51 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 10048456 |
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| DOI: | 10.13590/j.cjfh.2022.04.018 |