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Multivariate modular metabolic engineering and medium optimization for vitamin B12 production by Escherichia coli

التفاصيل البيبلوغرافية
العنوان: Multivariate modular metabolic engineering and medium optimization for vitamin B12 production by Escherichia coli
المؤلفون: Feitao Chen, Huan Fang, Jianghua Zhao, Pingtao Jiang, Huina Dong, Ying Zhao, Huiying Wang, Tongcun Zhang, Dawei Zhang
المصدر: Synthetic and Systems Biotechnology, Vol 9, Iss 3, Pp 453-461 (2024)
بيانات النشر: KeAi Communications Co., Ltd., 2024.
سنة النشر: 2024
المجموعة: LCC:Biotechnology
LCC:Biology (General)
مصطلحات موضوعية: Vitamin B12, Multivariate modular metabolic engineering, E. coli, Organic nitrogen sources, Fed-batch fermentation, Biotechnology, TP248.13-248.65, Biology (General), QH301-705.5
الوصف: Vitamin B12 is a complex compound synthesized by microorganisms. The industrial production of vitamin B12 relies on specific microbial fermentation processes. E. coli has been utilized as a host for the de novo biosynthesis of vitamin B12, incorporating approximately 30 heterologous genes. However, a metabolic imbalance in the intricate pathway significantly limits vitamin B12 production. In this study, we employed multivariate modular metabolic engineering to enhance vitamin B12 production in E. coli by manipulating two modules comprising a total of 10 genes within the vitamin B12 biosynthetic pathway. These two modules were integrated into the chromosome of a chassis cell, regulated by T7, J23119, and J23106 promoters to achieve combinatorial pathway optimization. The highest vitamin B12 titer was attained by engineering the two modules controlled by J23119 and T7 promoters. The inclusion of yeast powder to the fermentation medium increased the vitamin B12 titer to 1.52 mg/L. This enhancement was attributed to the effect of yeast powder on elevating the oxygen transfer rate and augmenting the strain's isopropyl-β-d-1-thiogalactopyranoside (IPTG) tolerance. Ultimately, vitamin B12 titer of 2.89 mg/L was achieved through scaled-up fermentation in a 5-liter fermenter. The strategies reported herein will expedite the development of industry-scale vitamin B12 production utilizing E. coli.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2405-805X
Relation: http://www.sciencedirect.com/science/article/pii/S2405805X24000516; https://doaj.org/toc/2405-805X
DOI: 10.1016/j.synbio.2024.03.017
URL الوصول: https://doaj.org/article/a721a38d0fdf40d1b79f2665401a8f6b
رقم الأكسشن: edsdoj.721a38d0fdf40d1b79f2665401a8f6b
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:2405805X
DOI:10.1016/j.synbio.2024.03.017