دورية أكاديمية
Use of cytobrush for bacteriological and cytological diagnosis of endometritis in mares
| العنوان: | Use of cytobrush for bacteriological and cytological diagnosis of endometritis in mares |
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| المؤلفون: | Chiara Del Prete, Francesca Paola Nocera, Giuseppe Piegari, Veronica Palumbo, Luisa De Martino, Natascia Cocchia, Orlando Paciello, Chiara Montano, Maria Pia Pasolini |
| المصدر: | Veterinary World, Vol 17, Iss 2, Pp 398-406 (2024) |
| بيانات النشر: | Veterinary World, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Animal culture LCC:Veterinary medicine |
| مصطلحات موضوعية: | endometritis, field conditions, mare, microbiology brush, uterine sampling, Animal culture, SF1-1100, Veterinary medicine, SF600-1100 |
| الوصف: | Background and Aim: A combined microbial and cytological examination of uterine samples is the main diagnostic method for endometritis in mares. This study aimed to describe a procedure for using the same uterine cytobrush (CB) for both bacteriological and cytological evaluation. Materials and Methods: The procedure consists of rolling the CB onto a sterilized glass slide immediately after collection and before the transfer into a sterile saline solution. In Experiment 1, a comparison between bacteriological results of the cotton swab (CS) and CB or pellet was made in 10 mares; in Experiment 2, bacteriological and cytological results were compared between different processing methods of CB in 28 mares; in other 6 mares, a CB was processed for cytology only, to investigate the reasons for the low cellularity of the pellet. Results: The agreement between culture results from the CB and CS was evaluated, and a comparison between the cytological data obtained by different processing methods of CB was performed. The perfect agreement between the CB and CS microbiological results was found. The described procedure enables useful diagnostic smears for cytology. Moreover, the seeding of both the tip of CB and the saline solution used for the transport produced accurate bacteriological results. Conclusion: The protocol described in this study for the use of CB for both cytological and bacteriological analysis could be used for the diagnosis of endometritis. To maximize diagnostic sample quality, cytology slides must be prepared with meticulous care in the field to preserve cellular integrity and minimize artifacts. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0972-8988 2231-0916 |
| Relation: | https://www.veterinaryworld.org/Vol.17/February-2024/19.pdf; https://doaj.org/toc/0972-8988; https://doaj.org/toc/2231-0916 |
| DOI: | 10.14202/vetworld.2024.398-406 |
| URL الوصول: | https://doaj.org/article/722731b78ca1470fa7c5b7b35419c4e5 |
| رقم الأكسشن: | edsdoj.722731b78ca1470fa7c5b7b35419c4e5 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 09728988 22310916 |
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| DOI: | 10.14202/vetworld.2024.398-406 |