دورية أكاديمية
Genetically engineered mesenchymal stem cells as a nitric oxide reservoir for acute kidney injury therapy
| العنوان: | Genetically engineered mesenchymal stem cells as a nitric oxide reservoir for acute kidney injury therapy |
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| المؤلفون: | Haoyan Huang, Meng Qian, Yue Liu, Shang Chen, Huifang Li, Zhibo Han, Zhong-Chao Han, Xiang-Mei Chen, Qiang Zhao, Zongjin Li |
| المصدر: | eLife, Vol 12 (2023) |
| بيانات النشر: | eLife Sciences Publications Ltd, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Medicine LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | nitric oxide, gaseous signaling molecule, genetically engineered mesenchymal stem cell, acute kidney injury, controlled release, delivery, Medicine, Science, Biology (General), QH301-705.5 |
| الوصف: | Nitric oxide (NO), as a gaseous therapeutic agent, shows great potential for the treatment of many kinds of diseases. Although various NO delivery systems have emerged, the immunogenicity and long-term toxicity of artificial carriers hinder the potential clinical translation of these gas therapeutics. Mesenchymal stem cells (MSCs), with the capacities of self-renewal, differentiation, and low immunogenicity, have been used as living carriers. However, MSCs as gaseous signaling molecule (GSM) carriers have not been reported. In this study, human MSCs were genetically modified to produce mutant β-galactosidase (β-GALH363A). Furthermore, a new NO prodrug, 6-methyl-galactose-benzyl-oxy NONOate (MGP), was designed. MGP can enter cells and selectively trigger NO release from genetically engineered MSCs (eMSCs) in the presence of β-GALH363A. Moreover, our results revealed that eMSCs can release NO when MGP is systemically administered in a mouse model of acute kidney injury (AKI), which can achieve NO release in a precise spatiotemporal manner and augment the therapeutic efficiency of MSCs. This eMSC and NO prodrug system provides a unique and tunable platform for GSM delivery and holds promise for regenerative therapy by enhancing the therapeutic efficiency of stem cells. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2050-084X |
| Relation: | https://elifesciences.org/articles/84820; https://doaj.org/toc/2050-084X |
| DOI: | 10.7554/eLife.84820 |
| URL الوصول: | https://doaj.org/article/7252d3fe7ee54915842cf2d763854d1a |
| رقم الأكسشن: | edsdoj.7252d3fe7ee54915842cf2d763854d1a |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2050084X |
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| DOI: | 10.7554/eLife.84820 |