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Haemophilus parasuis cytolethal distending toxin induces cell cycle arrest and p53-dependent apoptosis.

التفاصيل البيبلوغرافية
العنوان: Haemophilus parasuis cytolethal distending toxin induces cell cycle arrest and p53-dependent apoptosis.
المؤلفون: Gang Li, Hui Niu, Yanhe Zhang, Yanling Li, Fang Xie, Paul R Langford, Siguo Liu, Chunlai Wang
المصدر: PLoS ONE, Vol 12, Iss 5, p e0177199 (2017)
بيانات النشر: Public Library of Science (PLoS), 2017.
سنة النشر: 2017
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: Haemophilus parasuis is the causative agent of Glasser's disease in pigs. Cytolethal distending toxin (CDT) is an important virulence factor of H. parasuis. It is composed of three subunits: CdtA, CdtB and CdtC and all were successfully expressed in soluble form in Escherichia coli when the signal peptides were removed. Purified CdtB had DNase activity, i.e. caused DNA double strand damage, in vitro and in vivo prior to cell arrest and apoptosis. Flow cytometry analysis showed CdtB alone could induce cell cycle arrest and apoptosis in PK-15 porcine kidney and pulmonary alveolar macrophage (PAM) cells, which could be enhanced by CdtA or/and CdtC. CDT holotoxin could lead to significant cell distension, G2 arrest and apoptotic death in PK-15 and PAM cells. The apoptosis induced by CDT holotoxin was significantly inhibited by pifithrin-α, which indicates that it is p53-dependent. The results suggest that H. parasuis CDT holotoxin is a major virulence factor.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1932-6203
Relation: http://europepmc.org/articles/PMC5436662?pdf=render; https://doaj.org/toc/1932-6203
DOI: 10.1371/journal.pone.0177199
URL الوصول: https://doaj.org/article/72838c0b8afb484980bc6369e309ef2a
رقم الأكسشن: edsdoj.72838c0b8afb484980bc6369e309ef2a
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19326203
DOI:10.1371/journal.pone.0177199