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Genome-Wide Analysis of a TaLEA-Introduced Transgenic Populus simonii × Populus nigra Dwarf Mutant

التفاصيل البيبلوغرافية
العنوان: Genome-Wide Analysis of a TaLEA-Introduced Transgenic Populus simonii × Populus nigra Dwarf Mutant
المؤلفون: Jing Jiang, Gui-Feng Liu, Hui-Yu Li, Lin Lin, Rui Wei, Su Chen, Hong-Mei Yuan
المصدر: International Journal of Molecular Sciences, Vol 13, Iss 3, Pp 2744-2762 (2012)
بيانات النشر: MDPI AG, 2012.
سنة النشر: 2012
المجموعة: LCC:Biology (General)
LCC:Chemistry
مصطلحات موضوعية: dwarf, mutant, poplar, microarrays, AP2, RAV, Biology (General), QH301-705.5, Chemistry, QD1-999
الوصف: A dwarf mutant (dwf1) was obtained among 15 transgenic lines, when TaLEA (Tamarix androssowii late embryogenesis abundant gene) was introduced into Populus simonii × Populus nigra by Agrobacterium tumefaciens-mediated transformation. Under the same growth conditions, dwf1 height was significantly reduced compared with the wild type and the other transgenic lines. Because only one transgenic line (dwf1) displayed the dwarf phenotype, we considered that T-DNA insertion sites may play a role in the mutant formation. The mechanisms underlying this effect were investigated using TAIL-PCR (thermal asymmetric interlaced PCR) and microarrays methods. According to the TAIL-PCR results, two flanking sequences located on chromosome IV and VIII respectively, were cloned. The results indicated the integration of two independent T-DNA copies. We searched for the potential genes near to the T-DNA insertions. The nearest gene was a putative poplar AP2 transcription factor (GI: 224073210). Expression analysis showed that AP2 was up-regulated in dwf1 compared with the wild type and the other transgenic lines. According to the microarrays results, a total of 537 genes involved in hydrolase, kinase and transcription factor activities, as well as protein and nucleotide binding, showed significant alterations in gene expression. These genes were expressed in more than 60 metabolic pathways, including starch, sucrose, galactose and glycerolipid metabolism and phenylpropanoids and flavonoid biosyntheses. Our transcriptome and T-DNA insertion sites analyses might provide some useful insights into the dwarf mutant formation.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1422-0067
Relation: http://www.mdpi.com/1422-0067/13/3/2744/; https://doaj.org/toc/1422-0067
DOI: 10.3390/ijms13032744
URL الوصول: https://doaj.org/article/c7295bca74294e3e8e82d818539ff290
رقم الأكسشن: edsdoj.7295bca74294e3e8e82d818539ff290
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:14220067
DOI:10.3390/ijms13032744