دورية أكاديمية
The efficacy of immediate versus delayed antibiotic administration on bacterial growth and biofilm production of selected strains of uropathogenic Escherichia coli and Pseudomonas aeruginosa
| العنوان: | The efficacy of immediate versus delayed antibiotic administration on bacterial growth and biofilm production of selected strains of uropathogenic Escherichia coli and Pseudomonas aeruginosa |
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| المؤلفون: | Leah Gandee, Jer-Tsong Hsieh, Vanessa Sperandio, Cristiano G. Moreira, Chih-Ho Lai, Philippe E. Zimmern |
| المصدر: | International Brazilian Journal of Urology, Vol 41, Iss 1, Pp 67-77 (2015) |
| بيانات النشر: | Sociedade Brasileira de Urologia, 2015. |
| سنة النشر: | 2015 |
| المجموعة: | LCC:Diseases of the genitourinary system. Urology |
| مصطلحات موضوعية: | urinary tract infection, antibiotic therapy, biofilm formation, bacterial growth, Diseases of the genitourinary system. Urology, RC870-923 |
| الوصف: | Purpose The treatment of urinary tract infections (UTI) with antibiotics is commonly used, but recurrence and antibiotic resistance have been growing and concerning clinicians. We studied whether the rapid onset of a protective biofilm may be responsible for the lack of effectiveness of antibiotics against selected bacteria. Materials and Methods Two established uropathogenic Escherichia coli strains, UTI89 and CFT073, and two Pseudomonas aeruginosa strains, PA01 and Boston-41501, were studied to establish a reliable biofilm formation process. Bacterial growth (BG) was determined by optical density at 600 nm (OD 600) using a spectrophotometer, while biofilm formation (BF) using crystal violet staining was measured at OD 550. Next, these bacterial strains were treated with clinically relevant antibiotics, ciprofloxacin HCl (200 ng/mL and 2 μg/mL), nitrofurantoin (20 μg/mL and 40 μg/mL) and ampicillin (50 μg/mL) at time points of 0 (T0) or after 6 hours of culture (T6). All measurements, including controls (bacteria -1% DMSO), were done in triplicates and repeated three times for consistency. Results The tested antibiotics effectively inhibited both BG and BF when administered at T0 for UPEC strains, but not when the antibiotic administration started 6 hours later. For Pseudomonas strains, only Ciprofloxacin was able to significantly inhibit bacterial growth at T0 but only at the higher concentration of 2 μg/mL for T6. Conclusion When established UPEC and Pseudomonas bacteria were allowed to culture for 6 hours before initialization of treatment, the therapeutic effect of selected antibiotics was greatly suppressed when compared to immediate treatment, probably as a result of the protective nature of the biofilm. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1677-6119 1677-5538 |
| Relation: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382015000100067&lng=en&tlng=en; https://doaj.org/toc/1677-6119 |
| DOI: | 10.1590/S1677-5538.IBJU.2015.01.10 |
| URL الوصول: | https://doaj.org/article/72a861f919264c6397ccdedb62ba56bb |
| رقم الأكسشن: | edsdoj.72a861f919264c6397ccdedb62ba56bb |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 16776119 16775538 |
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| DOI: | 10.1590/S1677-5538.IBJU.2015.01.10 |