دورية أكاديمية
Dose-response effect of crude extracts produced by actinobacteria on in vitro rumen fermentation
| العنوان: | Dose-response effect of crude extracts produced by actinobacteria on in vitro rumen fermentation |
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| المؤلفون: | Bruna Gomes Alves, Cristian Marlon de Magalhães Rodrigues Martins, Marcos André Arcari, Ana Paula Ferranti Peti, Luiz Alberto Beraldo de Moraes, Marcos Veiga dos Santos |
| المصدر: | Brazilian Journal of Veterinary Research and Animal Science, Vol 55, Iss 3 (2018) |
| بيانات النشر: | Universidade de São Paulo, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Animal culture |
| مصطلحات موضوعية: | Actinobacteria, Ionophores, In vitro fermentation, Extracts, Animal culture, SF1-1100 |
| الوصف: | Actinobacteria have been researched as a source that produces crude extracts, which contain bioactive compounds able to act as antimicrobial agents. The present investigation evaluated the dose-response effect of two crude extracts, obtained at Caatinga rhizosphere (Caat) and Rhizophora mangle (AMC), on in vitro ruminal fermentation by: cumulative gas production, digestibility of dry (IVDMD) and organic matter (IVOMD), and short-chain fatty acids concentration (SCFA). Three multiparous Holstein dairy cows with ruminal fistula were used as the inoculum donors and fed a basal diet consisting of corn silage, soybean meal, urea, ground corn and mineral supplement. Ruminal fluid samples were incubated in glass bottles with 1 g of the dried and milled diet, a buffer solution, and the crude extracts evaluated in four doses (0.3, 0.6, 0.9 and 1.20 mg/10 mL inoculum) in a randomized block design, and the donators were considered as blocks with random effects. Additionally, negative controls were used. The results were expressed as average values based on triplicate analyses. Decreased cumulative gas production was observed according to linear dose response at 24, 48 and 72 h of incubation with the addition of Caat extract. The IVOMD showed a linear decrease at 72 h of incubation with dose Caat inclusion. Furthermore, the inclusion of Caat extract linearly reduced butyric and isovaleric acid concentrations, as well as acetate:propionate ratio. Finally, the Caat inclusion increased the propionic acid concentration in comparison to AMC extract. However, the inclusion of AMC extract did not affect any of the analyzed variables at the used doses. The Caat extract could be used as a modulator of in vitro ruminal fermentation, since it reduced acetate:propionate ratio and cumulative gas production. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Portuguese |
| تدمد: | 1678-4456 1413-9596 |
| Relation: | http://www.revistas.usp.br/bjvras/article/view/141243; https://doaj.org/toc/1413-9596; https://doaj.org/toc/1678-4456 |
| DOI: | 10.11606/issn.1678-4456.bjvras.2018.141243 |
| URL الوصول: | https://doaj.org/article/72c03f8c606445778074760984da4c43 |
| رقم الأكسشن: | edsdoj.72c03f8c606445778074760984da4c43 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 16784456 14139596 |
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| DOI: | 10.11606/issn.1678-4456.bjvras.2018.141243 |