دورية أكاديمية
Effect of exogenous gonadotropin on the transcriptome of human granulosa cells and follicular fluid hormone profiles
| العنوان: | Effect of exogenous gonadotropin on the transcriptome of human granulosa cells and follicular fluid hormone profiles |
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| المؤلفون: | Cui-Ling Lu, Zhi-Qiang Yan, Xue-Ling Song, Yang-Ying Xu, Xiao-Ying Zheng, Rong Li, Ping Liu, Huai-Liang Feng, Jie Qiao |
| المصدر: | Reproductive Biology and Endocrinology, Vol 17, Iss 1, Pp 1-9 (2019) |
| بيانات النشر: | BMC, 2019. |
| سنة النشر: | 2019 |
| المجموعة: | LCC:Gynecology and obstetrics LCC:Reproduction |
| مصطلحات موضوعية: | Granulosa cells, Transcriptome, Follicular fluid, Gonadotropins, Natural cycle, Gynecology and obstetrics, RG1-991, Reproduction, QH471-489 |
| الوصف: | Abstract Background Superovulation treatment had some adverse effects on maturity and development of oocytes. Can superovulation dose of gonadotropins (Gns) affect the transcriptome of granulosa cells and follicular fluid (FF) hormone levels? Methods One leading pre-ovulatory follicle per subject was used from three natural-cycle and four Gn-stimulated patients. Granulosa cells and FF samples were collected from the same leading follicle of each patient. RNA was extracted from granulosa cells and subjected to deep sequencing and analysis. Follicle-stimulating hormone (FSH), estradiol (E2), androstenedione (AND), testosterone (T), luteinizing hormone (LH), and progesterone (P4) levels in FF were measured by immunoassays. Student’s t test was used for statistical analysis. Results A total of 715 genes were up-regulated, and 287 genes were down-regulated, in the Gn-stimulated group relative to the control group. Gene Ontology analysis revealed that the down-regulated genes were enriched in cell cycle and meiosis pathways, primarily those associated with follicle or oocyte maturation and quality. On the other hand, the up-regulated genes were enriched in functions related to immunity and cytokine–cytokine receptor interactions. Compared to the follicles of natural cycle, the E2 and LH concentrations were significantly reduced (P |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1477-7827 |
| Relation: | http://link.springer.com/article/10.1186/s12958-019-0489-4; https://doaj.org/toc/1477-7827 |
| DOI: | 10.1186/s12958-019-0489-4 |
| URL الوصول: | https://doaj.org/article/7311e83e60c340e78b0068f042d880b4 |
| رقم الأكسشن: | edsdoj.7311e83e60c340e78b0068f042d880b4 |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 14777827 |
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| DOI: | 10.1186/s12958-019-0489-4 |