The grain faba bean (Vicia faba) which belongs to the family of the Leguminosae, is a crop that is grown worldwide for consumption by humans and livestock. Despite being a rich source of plant-based protein and various agro-ecological advantages its usage is limited due to its anti-nutrients in the form of the seed-compounds vicine and convicine (V+C). While markers for a low V+C content exist the underlying pathway and the responsible genes have remained unknown for a long time and only recently a possible pathway and enzyme were found. Genetic research into Vicia faba is difficult due to the lack of a reference genome and the near exclusivity of V+C to the species. Here, we present sequence reads obtained through genotyping-by-sequencing of 20 Vicia faba lines with varying V+C contents. For each line, ∼3 million 150 bp paired end reads are available. This data can be useful in the genomic research of Vicia faba in general and its V+C content in particular.
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