Objective: To investigate the impact of duration of controlled ovarian stimulation on vitro fertilization outcomes. Study Design: Medical records of 3194 IVF and ICSI cycles from the assisted reproduction unit of a tertiary hospital were retrospectively analyzed using a computer based database. Characteristics of cycles with duration of stimulation ≤ 8 days (group I), 9 to 12 days (group II) or ≥13 days (group III) were studied retrospectively. Subgroup analysis was also done for poor responders, patients with polycystic ovary syndrome and normoresponders. Results: Estradiol on trigger day, number of retrieved oocytes, mature oocytes and fertilized oocytes were significantly higher in group II compared to the other two groups when all cycles were analyzed (p