دورية أكاديمية
Determination of 25OHD in Serum Based on Quadrupole-Linear Ion Trap Tandem Mass Spectrometric Technique
| العنوان: | Determination of 25OHD in Serum Based on Quadrupole-Linear Ion Trap Tandem Mass Spectrometric Technique |
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| المؤلفون: | XIE Jie, YI Ke-ke, GAO Wen-jing, LIU Hao, LI Jia-lian, HUANG Ze-jian, LIU Mei-ying, YU Qing-ni, WANG Han-wen, JIANG You, DAI Xin-hua, FANG Xiang |
| المصدر: | Zhipu Xuebao, Vol 44, Iss 1, Pp 13-24 (2023) |
| بيانات النشر: | Editorial Board of Journal of Chinese Mass Spectrometry Society, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Chemistry |
| مصطلحات موضوعية: | liquid chromatography tandem mass spectrometry (lc-ms/ms), isotope dilution mass spectrometry, quadrupole, linear ion trap, 25ohd, Chemistry, QD1-999 |
| الوصف: | Vitamin D deficiency and insufficiency is a global health issue that afflicts people of different age groups all over the world. As a reliable indicator for the evaluation of vitamin D, 25OHD is usually determined by LC-MS/MS. The tandem mass spectrometry based on ion trap has the characteristics of simple structure and high sensitivity. However, there are some problems such as narrow linear range and space charge effect, which limit its analysis of trace substances in complex matrix samples. In this study, the device and technology of a new quadrupole linear ion trap mass spectrometry was used to reduce the space charge interference of trace or ultra trace target ions by high abundance impurity ions in complex matrices. For the first time, isotope dilution mass spectrometry for accurate measurement of serum 25OHD was established by QLIT-6610MD, which is quadrupole-linear ion trap tandem mass spectrometer, and was independently developed by Technology Innovation Center of Mass Spectrometry for State Market Regulation, Center for Advanced Measurement Science, National Institute of Metrology. Serum samples were precipitated with methanol, extracted with n-hexane, dried with liquid nitrogen, redissolved in the initial mobile phase, and determined by QLIT-6610MD. The matrix-matched standard curve was used for quantification. The linear ranges of 25OHD2 and 25OHD3 were in the range of 0.78-50 μg/L and 1.56-100 μg/L with R2>0.999 9, respectively. The detection and quantification limits of 25OHD2 and 25OHD3 were 0.06, 0.12 μg/L and 0.02, 0.06 μg/L, respectively. The precision of quality control was better than 4.06%. The recoveries ranged from 99.25% to 108.38%, and the coefficients of variation were all less than 5.1%. After the method confirmation, the quantitative results of clinical samples were compared with triple quadrupole tandem mass spectrometry. 50 Clinical serum samples were measured and compared by QLIT-6610MD, AB 4500MD and YS EXACT 9900MD. The correlation and consistency of the data were analyzed by Pearson and Bland-Altman. The correlation coefficient of the three groups data was R≥0.998 0, P |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Chinese |
| تدمد: | 1004-2997 |
| Relation: | http://www.jcmss.com.cn/CN/article/openArticlePDF.jsp?attachType=PDF&id=3118; https://doaj.org/toc/1004-2997 |
| DOI: | 10.7538/zpxb.2022.0103 |
| URL الوصول: | https://doaj.org/article/73c468f59a2f47bf91e4940019d6b9e5 |
| رقم الأكسشن: | edsdoj.73c468f59a2f47bf91e4940019d6b9e5 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 10042997 |
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| DOI: | 10.7538/zpxb.2022.0103 |