Function-specific IL-17A and dexamethasone interactions in primary human airway epithelial cells

التفاصيل البيبلوغرافية
العنوان:	Function-specific IL-17A and dexamethasone interactions in primary human airway epithelial cells
المؤلفون:	Siti Farah Rahmawati, Rémon Vos, I. Sophie T. Bos, Huib A. M. Kerstjens, Loes E. M. Kistemaker, Reinoud Gosens
المصدر:	Scientific Reports, Vol 12, Iss 1, Pp 1-13 (2022)
بيانات النشر:	Nature Portfolio, 2022.
سنة النشر:	2022
المجموعة:	LCC:Medicine LCC:Science
مصطلحات موضوعية:	Medicine, Science
الوصف:	Abstract Asthmatics have elevated levels of IL-17A compared to healthy controls. IL-17A is likely to contribute to reduced corticosteroid sensitivity of human airway epithelium. Here, we aimed to investigate the mechanistic underpinnings of this reduced sensitivity in more detail. Differentiated primary human airway epithelial cells (hAECs) were exposed to IL-17A in the absence or presence of dexamethasone. Cells were then collected for RNA sequencing analysis or used for barrier function experiments. Mucus was collected for volume measurement and basal medium for cytokine analysis. 2861 genes were differentially expressed by IL-17A (Padj
نوع الوثيقة:	article
وصف الملف:	electronic resource
اللغة:	English
تدمد:	2045-2322
Relation:	https://doaj.org/toc/2045-2322
DOI:	10.1038/s41598-022-15393-2
URL الوصول:	https://doaj.org/article/743b1d96993347b7928470e65d4f95bf
رقم الأكسشن:	edsdoj.743b1d96993347b7928470e65d4f95bf
قاعدة البيانات:	Directory of Open Access Journals

الوصف
تدمد:	20452322
DOI:	10.1038/s41598-022-15393-2