دورية أكاديمية
Rising of intracellular NAD+ level and oppositely directed changes in CD38 expression in hippocampal cells in experimental Alzheimer’s disease
| العنوان: | Rising of intracellular NAD+ level and oppositely directed changes in CD38 expression in hippocampal cells in experimental Alzheimer’s disease |
|---|---|
| المؤلفون: | A. A. Semenova, Ya. V. Gorina, E. D. Khilazheva, E. V. Kharitonova, A. B. Salmina |
| المصدر: | Сибирский научный медицинский журнал, Vol 41, Iss 5, Pp 37-46 (2021) |
| بيانات النشر: | Russian Academy of Sciences, Siberian Branch Publishing House, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Medicine |
| مصطلحات موضوعية: | alzheimer’s disease, beta amyloid, angiopathy, nad+, cd38, Medicine |
| الوصف: | The aim of the study was to assess the level of NAD+ in the brain of mice treated with beta-amyloid (Aβ), as well as to determine the activity of ADP-ribosyl cyclase/CD38 and the number of CD38-immunopositive neurons, astrocytes and endothelial cells. Material and methods. The Alzheimer’s disease model was reproduced by intrahippocampal administration of Aβ to C57BL/6 mice. Determination of the NAD+ level in the extracellular fluid of the brain and in the hippocampal tissue was carried out by spectrophotometric analysis. Evaluation of the enzymatic activity of ADP-ribosyl cyclase / CD38 was carried out by the fluorimetric method, determination of the number of CD38-immunopositive cells by the immunohistochemistry method. Results and discussion. The level of NAD+ was significantly increased in the hippocampal tissue in mice after administration of Aβ, while the level of extracellular NAD+ did not change. The activity of ADP-ribosyl cyclase / CD38 in the hippocampal tissue did not change, but the number of CD38-immunopositive neurons decreased, and the number of CD38+ endothelial cells increased in the hippocampus of mice after administration of Aβ. Conclusion. Opposite changes in the expression of ADP-ribosyl cyclase / CD38 in neurons and endotheliocytes correspond to different metabolic states of these types of cells and, along with an increased intracellular pool of NAD+ in experimental Alzheimer’s disease, reflect an adaptive stress response to Aβ administration. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | Russian |
| تدمد: | 2410-2512 2410-2520 |
| Relation: | https://sibmed.elpub.ru/jour/article/view/661; https://doaj.org/toc/2410-2512; https://doaj.org/toc/2410-2520 |
| DOI: | 10.18699/SSMJ20210505 |
| URL الوصول: | https://doaj.org/article/7480d16388184c92bc9266f10a59e9af |
| رقم الأكسشن: | edsdoj.7480d16388184c92bc9266f10a59e9af |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 24102512 24102520 |
|---|---|
| DOI: | 10.18699/SSMJ20210505 |