دورية أكاديمية
Determining the Relative Fitness Score of Mutant Viruses in a Population Using Illumina Paired-end Sequencing and Regression Analysis
| العنوان: | Determining the Relative Fitness Score of Mutant Viruses in a Population Using Illumina Paired-end Sequencing and Regression Analysis |
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| المؤلفون: | Hangfei Qi, C. Olson, Nicholas Wu, Yushen Du, Ren Sun |
| المصدر: | Bio-Protocol, Vol 5, Iss 10 (2015) |
| بيانات النشر: | Bio-protocol LLC, 2015. |
| سنة النشر: | 2015 |
| المجموعة: | LCC:Biology (General) |
| مصطلحات موضوعية: | Biology (General), QH301-705.5 |
| الوصف: | Recent advances in DNA sequencing capacity to accurately quantify the copy number of individual variants in a large and diverse population allows in parallel determination of the phenotypic effects caused by each genetic modification. This systematic profiling approach is a combination of forward and reverse genetics, which we refer to as quantitative high-resolution genetics (qHRG). This protocol describes how to determine the relative fitness score of each variant compared to wild type (WT) virus based on its frequency determined by Illumina sequencing. Random mutagenesis techniques will be used to introduce randomization at each codon position of the targeted region, thereby generating a comprehensive input mutant library with substitutions at each position of interest (Qi et al., 2014; Wu et al., 2014a; Wu et al., 2014b). After selection, each selected library will be sequenced by Illumina paired-end sequencing and the frequency of each mutation will be determined. Based on the change in frequency, the relative fitness score of each mutant can be calculated with regression analysis. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2331-8325 14593459 |
| Relation: | https://bio-protocol.org/en/bpdetail?id=1475&type=0; https://doaj.org/toc/2331-8325 |
| DOI: | 10.21769/BioProtoc.1475 |
| URL الوصول: | https://doaj.org/article/74d29cab24e74ef98c1459345958643f |
| رقم الأكسشن: | edsdoj.74d29cab24e74ef98c1459345958643f |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 23318325 14593459 |
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| DOI: | 10.21769/BioProtoc.1475 |