Rice is one of the most important crops in the world; meanwhile, the rice field is also an important contributor to greenhouse gas methane emission. Therefore, it is important to get an accurate estimation of rice acreage for both food production and climate change related studies. The eastern plain region is one of the major single-cropped rice (SCR) growing areas in China. Subjected to the topography and intensified human activities, the rice fields are generally fragmented and irregular. How remote sensing can meet this challenge to accurately estimate the acreage of the rice in this region using medium-resolution imagery is the topic of this study. In this study, the applicability of the Chinese HJ-1A/B satellites and a two-band enhanced vegetation index (EVI2) was investigated. Field campaigns were carried out during the rice growing season and ground-truth data were collected for classification accuracy assessments in 2012. A stepwise classification strategy utilizing the EVI2 signatures during key phenology stages, i.e., the transplanting and the vegetative to reproductive transition phases, of the SCR was proposed, and the overall classification accuracy was 91.7%. The influence of the mixed pixel and boundary effects to classification accuracy was also investigated. This work demonstrates that the Chinese HJ-1A/B data are suitable data source to estimating SCR cropping area under complex land cover composition.