دورية أكاديمية
Coumarin and eugenol ameliorate LPS-induced inflammation in RAW 264.7 cells via modulating the NLRP3 inflammasome pathway
| العنوان: | Coumarin and eugenol ameliorate LPS-induced inflammation in RAW 264.7 cells via modulating the NLRP3 inflammasome pathway |
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| المؤلفون: | Jyotsana Bakshi, Somnath Singh, KP Mishra |
| المصدر: | Asian Pacific Journal of Tropical Biomedicine, Vol 14, Iss 1, Pp 40-46 (2024) |
| بيانات النشر: | Wolters Kluwer Medknow Publications, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Arctic medicine. Tropical medicine LCC:Biology (General) |
| مصطلحات موضوعية: | inflammation, cytokines, coumarin, eugenol, nlrp3, lps, Arctic medicine. Tropical medicine, RC955-962, Biology (General), QH301-705.5 |
| الوصف: | Objective: To investigate the underlying mechanism of anti-inflammatory action of coumarin and eugenol in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Methods: RAW 264.7 cells were treated with 2.5 μg/mL of LPS, 50 μM of coumarin, and 50 μM eugenol for 24 h. The viability of the cells was assessed using MTT assay. The production of nitric oxide was determined using Griess reagent and DCFH-DA was used to measure the production of reactive oxygen species. The protein expression of NLRP3, IL-1β, NF-κB, and cyclooxygenase 2 was assessed using Western blot analysis. Results: Coumarin and eugenol showed anti-inflammatory effects against LPS-induced inflammatory response by ameliorating the expression of NLRP3 inflammasome and NF-κB, which further led to a subsequent reduction in IL-1β, nitric oxide, and reactive oxygen species. Conclusions: Coumarin and eugenol exert their anti-inflammatory activities by modulating the NLRP3 inflammasome pathway and NF-κB. These compounds may have promising therapeutic applications for the treatment of various inflammatory diseases. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2221-1691 2588-9222 |
| Relation: | http://www.apjtb.org/article.asp?issn=2221-1691;year=2024;volume=14;issue=1;spage=40;epage=46;aulast=Bakshi; https://doaj.org/toc/2221-1691; https://doaj.org/toc/2588-9222 |
| DOI: | 10.4103/2221-1691.393579 |
| URL الوصول: | https://doaj.org/article/7659632b9a4d4998882b0343f157f0a7 |
| رقم الأكسشن: | edsdoj.7659632b9a4d4998882b0343f157f0a7 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 22211691 25889222 |
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| DOI: | 10.4103/2221-1691.393579 |