دورية أكاديمية
Design and Characterization of a Peptide Mimotope of the HIV-1 gp120 Bridging Sheet
| العنوان: | Design and Characterization of a Peptide Mimotope of the HIV-1 gp120 Bridging Sheet |
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| المؤلفون: | Guido Poli, Giuseppe Scala, Ileana Quinto, Vincenzo Pavone, Piergiuseppe de Berardinis, Giulia Morsica, Maria Trovato, Jan Rafay, Boris Ferko, Concetta Andreozzi, Angela Lombardi, David Montefiori, Annarita Scialdone, Eleonora Vecchio, Annalisa Rossi, Camillo Palmieri, Antonio Pisano, Marilena Pontoriero, Enrico Iaccino, Selena Mimmi, Francesca Fasanella Masci, Cristina Falcone, Giuseppe Fiume, Antonella Caivano, Annamaria de Laurentiis, Marco Schiavone |
| المصدر: | International Journal of Molecular Sciences, Vol 13, Iss 5, Pp 5674-5699 (2012) |
| بيانات النشر: | MDPI AG, 2012. |
| سنة النشر: | 2012 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | HIV-1 vaccine, bridging sheet, mimotope, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | The Bridging Sheet domain of HIV-1 gp120 is highly conserved among the HIV-1 strains and allows HIV-1 binding to host cells via the HIV-1 coreceptors. Further, the bridging sheet domain is a major target to neutralize HIV-1 infection. We rationally designed four linear peptide epitopes that mimic the three-dimensional structure of bridging sheet by using molecular modeling. Chemically synthesized peptides BS3 and BS4 showed a fair degree of antigenicity when tested in ELISA with IgG purified from HIV+ broadly neutralizing sera while the production of synthetic peptides BS1 and BS2 failed due to their high degree of hydrophobicity. To overcome this limitation, we linked all four BS peptides to the COOH-terminus of GST protein to test both their antigenicity and immunogenicity. Only the BS1 peptide showed good antigenicity; however, no envelope specific antibodies were elicited upon mice immunization. Therefore we performed further analyses by linking BS1 peptide to the NH2-terminus of the E2 scaffold from the Geobacillus Stearothermophylus PDH complex. The E2-BS1 fusion peptide showed good antigenic results, however only one immunized rabbit elicited good antibody titers towards both the monomeric and oligomeric viral envelope glycoprotein (Env). In addition, moderate neutralizing antibodies response was elicited against two HIV-1 clade B and one clade C primary isolates. These preliminary data validate the peptide mimotope approach as a promising tool to obtain an effective HIV-1 vaccine. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 13055674 1422-0067 |
| Relation: | http://www.mdpi.com/1422-0067/13/5/5674; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms13055674 |
| URL الوصول: | https://doaj.org/article/77240e71c25747588f94c3eafd72e911 |
| رقم الأكسشن: | edsdoj.77240e71c25747588f94c3eafd72e911 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 13055674 14220067 |
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| DOI: | 10.3390/ijms13055674 |