دورية أكاديمية
mRNA expression profiling of mitochondrial subunits in subjects with Parkinson’s disease
| العنوان: | mRNA expression profiling of mitochondrial subunits in subjects with Parkinson’s disease |
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| المؤلفون: | Michele Salemi, Filomena Cosentino, Giuseppe Lanza, Mariagiovanna Cantone, Maria Grazia Salluzzo, Giorgio Giurato, Eugenia Borgione, Giovanna Marchese, Sandro Santa Paola, Bartolo Lanuzza, Corrado Romano, Raffaele Ferri |
| المصدر: | Archives of Medical Science, Vol 19, Iss 3, Pp 678-686 (2021) |
| بيانات النشر: | Termedia Publishing House, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Medicine |
| مصطلحات موضوعية: | parkinson’s disease, mitochondrial subunits, next generation sequencing, quantitative real-time polymerase chain reaction, Medicine |
| الوصف: | Introduction Parkinson’s disease (PD) is a common adult-onset neurodegenerative disorder caused by a progressive loss of dopaminergic neurons due to the accumulation of α-synuclein in the substantia nigra. Mitochondria are known to play a key role in cell respiratory function and bioenergetics. Indeed, mitochondrial dysfunction causes insufficient energy production required to satisfy the needs of several organs, especially the nervous system. However, the profiling of messenger RNA (mRNA) expression of mitochondrial subunits in PD has not been systematically investigated yet. Material and methods We explored the mRNA expression of mitochondrial DNA (mtDNA) encoded respiratory chain (RC) subunits in 43 PD patients and 43 normal controls (NC). Next generation sequencing analysis (NGS) was used and quantitative real-time polymerase chain reaction (qRT-PCR) assay was used for confirmation of the NGS results. Results All tested mitochondrial RC subunits were significantly over-expressed in subjects with PD compared to NC. In qRT-PCR the mean expression of all mitochondrial subunits had an expression level of at least 7 times compared to NC. Conclusions The over-expression of mitochondrial subunits in PD subjects with respect to NC might be secondary to a degeneration-related alteration of the mitochondrial structure and/or dynamics, or to the occurrence of a compensatory mechanism. The study of specific mRNA by peripheral blood mononuclear cells may provide a further diagnostic frame for early detection PD patients. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1734-1922 1896-9151 |
| Relation: | https://www.archivesofmedicalscience.com/mRNA-expression-profiling-of-mitochondrial-subunits-in-subjects-with-Parkinson-s,131629,0,2.html; https://doaj.org/toc/1734-1922; https://doaj.org/toc/1896-9151 |
| DOI: | 10.5114/aoms/131629 |
| URL الوصول: | https://doaj.org/article/e7728c5ae8434c2f9d8469dd775aaa7d |
| رقم الأكسشن: | edsdoj.7728c5ae8434c2f9d8469dd775aaa7d |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 17341922 18969151 |
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| DOI: | 10.5114/aoms/131629 |