دورية أكاديمية
Deoxynivalenol triggers mitotic catastrophe and apoptosis in C2C12 myoblasts
| العنوان: | Deoxynivalenol triggers mitotic catastrophe and apoptosis in C2C12 myoblasts |
|---|---|
| المؤلفون: | Zhenzhen Wang, Huimin Duan, Xue You, Qian Peng, Ningyang Yuan, Rula Sha, Zhiqin Xie, Ying Feng |
| المصدر: | Ecotoxicology and Environmental Safety, Vol 281, Iss , Pp 116607- (2024) |
| بيانات النشر: | Elsevier, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Environmental pollution LCC:Environmental sciences |
| مصطلحات موضوعية: | Deoxynivalenol treatment, Skeletal muscle cells, Toxicity mechanism, Mitotic defects, Apoptosis, Environmental pollution, TD172-193.5, Environmental sciences, GE1-350 |
| الوصف: | Deoxynivalenol (DON), commonly known as vomitoxin, is a mycotoxin produced by fungi and is frequently found as a contaminant in various cereal-based food worldwide. While the harmful effects of DON have been extensively studied in different tissues, its specific impact on the proliferation of skeletal muscle cells remains unclear. In this study, we utilized murine C2C12 myoblasts as a model to explore the influence of DON on their proliferation. Our observations indicated that DON exhibits dose-dependent toxicity, significantly inhibiting the proliferation of C2C12 cells. Through the application of RNA-seq analysis combined with gene set enrichment analysis, we identified a noteworthy downregulation of genes linked to the extracellular matrix (ECM) and condensed chromosome. Concurrently with the reduced expression of ECM genes, immunostaining analysis revealed notable changes in the distribution of fibronectin, a vital ECM component, condensing into clusters and punctate formations. Remarkably, the exposure to DON induced the formation of multipolar spindles, leading to the disruption of the normal cell cycle. This, in turn, activated the p53-p21 signaling pathway and ultimately resulted in apoptosis. These findings contribute significant insights into the mechanisms through which DON induces toxicity within skeletal muscle cells. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0147-6513 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S0147651324006833; https://doaj.org/toc/0147-6513 |
| DOI: | 10.1016/j.ecoenv.2024.116607 |
| URL الوصول: | https://doaj.org/article/775a06eca35f42eca961e0184a82948e |
| رقم الأكسشن: | edsdoj.775a06eca35f42eca961e0184a82948e |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 01476513 |
|---|---|
| DOI: | 10.1016/j.ecoenv.2024.116607 |