دورية أكاديمية
Cell death induced by tamoxifen in human blood lymphocytes cultivated in vitro - doi: 10.4025/actascibiolsci.v32i4.7015
| العنوان: | Cell death induced by tamoxifen in human blood lymphocytes cultivated in vitro - doi: 10.4025/actascibiolsci.v32i4.7015 |
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| المؤلفون: | Naila Francis Paulo de Oliveira, Selma Candelária Genari, Heidi Dolder |
| المصدر: | Acta Scientiarum: Biological Sciences, Vol 32, Iss 4 (2010) |
| بيانات النشر: | Universidade Estadual de Maringá, 2010. |
| سنة النشر: | 2010 |
| المجموعة: | LCC:Biology (General) LCC:Microbiology |
| مصطلحات موضوعية: | Apoptosis, autophagy, chemotherapeutic, cytotoxicity, morphology, ultrastructure, Biology (General), QH301-705.5, Microbiology, QR1-502 |
| الوصف: | Many chemotherapeutic agents with a potential against solid tumors or leukemia can cause lymphopenia. Tamoxifen (TAM) is a synthetic non-steroidal anti-estrogen drug employed in female breast cancer treatment. The present study investigated the capacity of TAM to induce cell death in human lymphocytes cultivated in vitro. Lymphocytes were obtained from young (25-30 years; n = 3) and elderly women (58-77 years; n = 3) and cultivated for 24 or 48h, with or without TAM (20 µM). After the culture, cell viability, immunocytochemical response and ultrastructure were evaluated. TAM affected lymphocytes in a time- dependent manner, and cells obtained from elderly women were the most sensitive to TAM. Immunocytochemical analysis evidenced higher frequency of apoptosis in treated cells, and the ultrastructural study revealed autophagic vacuoles, differing from the controls. In summary, the treated lymphocytes were affected by TAM, leading to cell death by apoptosis and autophagy. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1679-9283 1807-863X |
| Relation: | https://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/7015; https://doaj.org/toc/1679-9283; https://doaj.org/toc/1807-863X |
| DOI: | 10.4025/actascibiolsci.v32i4.7015 |
| URL الوصول: | https://doaj.org/article/77645dc250964e2bb912f4d7907c874e |
| رقم الأكسشن: | edsdoj.77645dc250964e2bb912f4d7907c874e |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 16799283 1807863X |
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| DOI: | 10.4025/actascibiolsci.v32i4.7015 |