دورية أكاديمية
أعرض في EDS

Embryonic morphogen nodal is associated with progression and poor prognosis of hepatocellular carcinoma.

التفاصيل البيبلوغرافية
العنوان: Embryonic morphogen nodal is associated with progression and poor prognosis of hepatocellular carcinoma.
المؤلفون: Jing Chen, Wen-Bin Liu, Wei-Dong Jia, Ge-Liang Xu, Jin-Liang Ma, Yun Ren, Hao Chen, Si-Nan Sun, Mei Huang, Jian-Sheng Li
المصدر: PLoS ONE, Vol 9, Iss 1, p e85840 (2014)
بيانات النشر: Public Library of Science (PLoS), 2014.
سنة النشر: 2014
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: BACKGROUND: Nodal, a TGF-β-related embryonic morphogen, is involved in multiple biologic processes. However, the expression of Nodal in hepatocellular carcinoma (HCC) and its correlation with tumor angiogenesis, epithelial-mesenchymal transition, and prognosis is unclear. METHODS: We used real-time PCR and Western blotting to investigate Nodal expression in 6 HCC cell lines and 1 normal liver cell line, 16 pairs of tumor and corresponding paracarcinomatous tissues from HCC patients. Immunohistochemistry was performed to examine Nodal expression in HCC and corresponding paracarcinomatous tissues from 96 patients. CD34 and Vimentin were only examined in HCC tissues of patients mentioned above. Nodal gene was silenced by shRNA in MHCC97H and HCCLM3 cell lines, and cell migration and invasion were detected. Statistical analyses were applied to evaluate the prognostic value and associations of Nodal expression with clinical parameters. RESULTS: Nodal expression was detected in HCC cell lines with high metastatic potential alone. Nodal expression is up-regulated in HCC tissues compared with paracarcinomatous and normal liver tissues. Nodal protein was expressed in 70 of the 96 (72.9%) HCC tumors, and was associated with vascular invasion (P = 0.000), status of metastasis (P = 0.004), AFP (P = 0.049), ICGR15 (indocyanine green retention rate at 15 min) (P = 0.010) and tumor size (P = 0.000). High Nodal expression was positively correlated with high MVD (microvessal density) (P = 0.006), but not with Vimentin expression (P = 0.053). Significantly fewer migrated and invaded cells were seen in shRNA group compared with blank group and negative control group (P
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1932-6203
Relation: http://europepmc.org/articles/PMC3897529?pdf=render; https://doaj.org/toc/1932-6203
DOI: 10.1371/journal.pone.0085840
URL الوصول: https://doaj.org/article/77c6f093ac6843ca8e6c4f5e1349b3a8
رقم الأكسشن: edsdoj.77c6f093ac6843ca8e6c4f5e1349b3a8
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19326203
DOI:10.1371/journal.pone.0085840