دورية أكاديمية
LncRNA CRNDE attenuates chemoresistance in gastric cancer via SRSF6-regulated alternative splicing of PICALM
| العنوان: | LncRNA CRNDE attenuates chemoresistance in gastric cancer via SRSF6-regulated alternative splicing of PICALM |
|---|---|
| المؤلفون: | Feifei Zhang, Hui Wang, Jiang Yu, Xueqing Yao, Shibin Yang, Weidong Li, Lijun Xu, Liang Zhao |
| المصدر: | Molecular Cancer, Vol 20, Iss 1, Pp 1-6 (2021) |
| بيانات النشر: | BMC, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens |
| مصطلحات موضوعية: | Gastric cancer, Long noncoding RNA CRNDE, Chemoresistant, Autophagy, Protein splicing, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282 |
| الوصف: | Abstract De novo and acquired resistance, which are mainly mediated by genetic alterations, are barriers to effective routine chemotherapy. However, the mechanisms underlying gastric cancer (GC) resistance to chemotherapy are still unclear. We showed that the long noncoding RNA CRNDE was related to the chemosensitivity of GC in clinical samples and a PDX model. CRNDE was decreased and inhibited autophagy flux in chemoresistant GC cells. CRNDE directly bound to splicing protein SRSF6 to reduce its protein stability and thus regulate alternative splicing (AS) events. We determined that SRSF6 regulated the PICALM exon 14 skip splice variant and triggered a significant S-to-L isoform switch, which contributed to the expression of the long isoform of PICALM (encoding PICALML). Collectively, our findings reveal the key role of CRNDE in autophagy regulation, highlighting the significance of CRNDE as a potential prognostic marker and therapeutic target against chemoresistance in GC. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1476-4598 13887645 |
| Relation: | https://doaj.org/toc/1476-4598 |
| DOI: | 10.1186/s12943-020-01299-y |
| URL الوصول: | https://doaj.org/article/781d50c285db4105ad13887645aa29ca |
| رقم الأكسشن: | edsdoj.781d50c285db4105ad13887645aa29ca |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 14764598 13887645 |
|---|---|
| DOI: | 10.1186/s12943-020-01299-y |