دورية أكاديمية
أعرض في EDS

A Next-Generation Sequencing of Plasma Exosome-Derived microRNAs and Target Gene Analysis with a Microarray Database of Thermally Injured Skins: Identification of Blood-to-Tissue Interactions at Early Burn Stage

التفاصيل البيبلوغرافية
العنوان: A Next-Generation Sequencing of Plasma Exosome-Derived microRNAs and Target Gene Analysis with a Microarray Database of Thermally Injured Skins: Identification of Blood-to-Tissue Interactions at Early Burn Stage
المؤلفون: Li SJ, Cai ZW, Yang HF, Tang XD, Fang X, Qiu L, Wang F, Chen XL
المصدر: Journal of Inflammation Research, Vol Volume 14, Pp 6783-6798 (2021)
بيانات النشر: Dove Medical Press, 2021.
سنة النشر: 2021
المجموعة: LCC:Pathology
LCC:Therapeutics. Pharmacology
مصطلحات موضوعية: burn, exosome, mirna, next-generation sequencing, bioinformatics, Pathology, RB1-214, Therapeutics. Pharmacology, RM1-950
الوصف: Shi-Ji Li,* Zhi-Wen Cai,* Hong-Fu Yang,* Xu-Dong Tang, Xiao Fang, Le Qiu, Fei Wang, Xu-Lin Chen Department of Burns, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xu-Lin ChenDepartment of Burns, The First Affiliated Hospital of Anhui Medical University, 120 Wanshui Road, Hefei, Anhui, 230088, People’s Republic of ChinaTel/Fax +86-551-65908495Email okcxl@126.comBackground: Plasma exosome-derived microRNA (miRNA) profiles following thermal injury and their relationship with gene expression derangements in burned skin remain unexplored. This study focused on the identification of key miRNA-mRNA axes in potential blood-to-tissue interactions at early burn stage.Methods: Plasma exosomes were obtained from 6 severe burn patients 4– 7 days post injury and 6 healthy volunteers. Next-generation sequencing (NGS) of exosomal small RNAs presented the differentially expressed miRNAs (DEMs). Target genes of the DEMs were predicted in the mirDIP database. Dataset GSE8056 was enrolled to acquire differentially expressed genes (DEGs) in burned skin compared to normal skin. Overlap between the DEGs and target genes of the DEMs were focus genes. The protein–protein interaction (PPI) network and enrichment analyses of the focus genes demonstrated hub genes and suggested underlying mechanisms and pathways. The hub genes and upstream DEMs were selected to construct key miRNA-mRNA axes.Results: The NGS of plasma exosome-derived small RNAs identified 85 DEMs (14 downregulated miRNAs and 71 upregulated miRNAs) with 12,901 predicted target genes. Dataset GSE8056 exhibited 1861 DEGs in partial-thickness burned skins 4– 7 days postburn. The overlap between DEGs and target genes of DEMs displayed 1058 focus genes. The top 9 hub genes (CDK1, CCNB1, CCNA2, BUB1B, PLK1, KIF11, AURKA, NUSAP1 and CDCA8) in the PPI network of the focus genes pointed to 16 upstream miRNAs in DEMs, including 4 downregulated miRNAs (hsa-miR-6848-3p, has-miR-4684-3p, has-miR-4786-5p and has-miR-365a-5p) and 12 upregulated miRNAs (hsa-miR-6751-3p, hsa-miR-718, hsa-miR-4754, hsa-miR-6754-3p, hsa-miR-4739, hsa-miR-6739-5p, hsa-miR-6884-3p, hsa-miR-1224-3p, hsa-miR-6878-3p, hsa-miR-6795-3p, hsa-miR-550a-3p, and hsa-miR-550b-3p). A key miRNA-mRNA network in potential blood-to-tissue interactions at early burn stage was therefore constructed.Conclusion: An NGS and bioinformatic analysis in the study identified key miRNA-mRNA axes in potential blood-to-tissue interactions at early burn stage, suggesting plasma exosome-derived miRNAs may impact on the alteration patterns of gene expressions in a burn wound.Keywords: burn, exosome, miRNA, next-generation sequencing, bioinformatics
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1178-7031
Relation: https://www.dovepress.com/a-next-generation-sequencing-of-plasma-exosome-derived-micrornas-and-t-peer-reviewed-fulltext-article-JIR; https://doaj.org/toc/1178-7031
URL الوصول: https://doaj.org/article/782e7134cfce4073923016c5a2a0c5f7
رقم الأكسشن: edsdoj.782e7134cfce4073923016c5a2a0c5f7
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:11787031