دورية أكاديمية
Generation and Imaging of Transgenic Mice that Express G-CaMP7 under a Tetracycline Response Element.
| العنوان: | Generation and Imaging of Transgenic Mice that Express G-CaMP7 under a Tetracycline Response Element. |
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| المؤلفون: | Masaaki Sato, Masako Kawano, Masamichi Ohkura, Keiko Gengyo-Ando, Junichi Nakai, Yasunori Hayashi |
| المصدر: | PLoS ONE, Vol 10, Iss 5, p e0125354 (2015) |
| بيانات النشر: | Public Library of Science (PLoS), 2015. |
| سنة النشر: | 2015 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | The spatiotemporally controlled expression of G-CaMP fluorescent calcium indicator proteins can facilitate reliable imaging of brain circuit activity. Here, we generated a transgenic mouse line that expresses G-CaMP7 under a tetracycline response element. When crossed with a forebrain-specific tetracycline-controlled transactivator driver line, the mice expressed G-CaMP7 in defined cell populations in a tetracycline-controlled manner, notably in pyramidal neurons in layer 2/3 of the cortex and in the CA1 area of the hippocampus; this expression allowed for imaging of the in vivo activity of these circuits. This mouse line thus provides a useful genetic tool for controlled G-CaMP expression in vivo. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1932-6203 |
| Relation: | http://europepmc.org/articles/PMC4422725?pdf=render; https://doaj.org/toc/1932-6203 |
| DOI: | 10.1371/journal.pone.0125354 |
| URL الوصول: | https://doaj.org/article/e78425588a324334a87e81d08215519d |
| رقم الأكسشن: | edsdoj.78425588a324334a87e81d08215519d |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0125354 |