دورية أكاديمية
Belt and braces: Two escape ways to maintain the cassette reservoir of large chromosomal integrons.
| العنوان: | Belt and braces: Two escape ways to maintain the cassette reservoir of large chromosomal integrons. |
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| المؤلفون: | Egill Richard, Baptiste Darracq, Eloi Littner, Gael A Millot, Valentin Conte, Thomas Cokelaer, Jan Engelstädter, Eduardo P C Rocha, Didier Mazel, Céline Loot |
| المصدر: | PLoS Genetics, Vol 20, Iss 4, p e1011231 (2024) |
| بيانات النشر: | Public Library of Science (PLoS), 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Genetics |
| مصطلحات موضوعية: | Genetics, QH426-470 |
| الوصف: | Integrons are adaptive devices that capture, stockpile, shuffle and express gene cassettes thereby sampling combinatorial phenotypic diversity. Some integrons called sedentary chromosomal integrons (SCIs) can be massive structures containing hundreds of cassettes. Since most of these cassettes are non-expressed, it is not clear how they remain stable over long evolutionary timescales. Recently, it was found that the experimental inversion of the SCI of Vibrio cholerae led to a dramatic increase of the cassette excision rate associated with a fitness defect. Here, we question the evolutionary sustainability of this apparently counter selected genetic context. Through experimental evolution, we find that the integrase is rapidly inactivated and that the inverted SCI can recover its original orientation by homologous recombination between two insertion sequences (ISs) present in the array. These two outcomes of SCI inversion restore the normal growth and prevent the loss of cassettes, enabling SCIs to retain their roles as reservoirs of functions. These results illustrate a nice interplay between gene orientation, genome rearrangement, bacterial fitness and demonstrate how integrons can benefit from their embedded ISs. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1553-7390 1553-7404 |
| Relation: | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1011231&type=printable; https://doaj.org/toc/1553-7390; https://doaj.org/toc/1553-7404 |
| DOI: | 10.1371/journal.pgen.1011231&type=printable |
| DOI: | 10.1371/journal.pgen.1011231 |
| URL الوصول: | https://doaj.org/article/7858995495e6436592d51e1f865adab5 |
| رقم الأكسشن: | edsdoj.7858995495e6436592d51e1f865adab5 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 15537390 15537404 |
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| DOI: | 10.1371/journal.pgen.1011231&type=printable |