The manuscript presents an algorithm for the optimal estimation of the amplitude and propagation delay time of an ultra-wideband radio signal, in systems for the passive location of fixed targets based on the hybrid RSS/TDoA method in two-dimensional space with two base stations. The optimal estimate is based on the Bayesian strategy of maximum a posteriori probability density, taking into account a priori data on the statistical properties of the Line of Sight radio channel during Gaussian monocycle propagation. The Bayesian Cramer–Rao lower bound (BCRLB) of the delay time and the amplitude estimates for a time-discrete signal are calculated, and the resulting parameter estimate is compared with BCRLB. An algorithm has been developed for optimal estimation of distances from the radiation source to base stations, based on the results of the measurements of the amplitude and the propagation delay time of the UWB radio signal. The calculation of the statistical characteristics of the obtained estimate is carried out, and the functional dependence of the characteristics on various parameters is analyzed.