دورية أكاديمية
Specification of Bacteriophage Isolated Against Clinical Methicillin-Resistant
| العنوان: | Specification of Bacteriophage Isolated Against Clinical Methicillin-Resistant |
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| المؤلفون: | Ahmad Nasser, Reza Azizian, Mohsen Tabasi, Jamil Kheirvari Khezerloo, Fatemah Sadeghpour Heravi, Morovat Taheri Kalani, Norkhoda Sadeghifard, Razieh Amini, Iraj Pakzad, Amin Radmanesh, Farid Azizi Jalilian |
| المصدر: | Osong Public Health and Research Perspectives, Vol 10, Iss 1, Pp 20-24 (2019) |
| بيانات النشر: | Korea Disease Control and Prevention Agency, 2019. |
| سنة النشر: | 2019 |
| المجموعة: | LCC:Special situations and conditions LCC:Infectious and parasitic diseases |
| مصطلحات موضوعية: | bacteriophage, methicillin-resistant, random amplified polymorphic deoxyribonucleic acid - polymerase chain reaction, scanning transmission electron microscopy, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Special situations and conditions, RC952-1245, Infectious and parasitic diseases, RC109-216 |
| الوصف: | Objectives The emergence of resistant bacteria is being increasingly reported around the world, potentially threatening millions of lives. Amongst resistant bacteria, methicillin-resistant Staphylococcus aureus (MRSA) is the most challenging to treat. This is due to emergent MRSA strains and less effective traditional antibiotic therapies to Staphylococcal infections. The use of bacteriophages (phages) against MRSA is a new, potential alternate therapy. In this study, morphology, genetic and protein structure of lytic phages against MRSA have been analysed. Methods Isolation of livestock and sewage bacteriophages were performed using 0.4 μm membrane filters. Plaque assays were used to determine phage quantification by double layer agar method. Pure plaques were then amplified for further characterization. Sulfate-polyacrylamide gel electrophoresis and random amplification of polymorphic DNA were run for protein evaluation, and genotyping respectively. Transmission electron microscope was also used to detect the structure and taxonomic classification of phage visually. Results Head and tail morphology of bacteriophages against MRSA were identified by transmission electron microscopy and assigned to the Siphoviridae family and the Caudovirales order. Conclusion Bacteriophages are the most abundant microorganism on Earth and coexist with the bacterial population. They can destroy bacterial cells successfully and effectively. They cannot enter mammalian cells which saves the eukaryotic cells from lytic phage activity. In conclusion, phage therapy may have many potential applications in microbiology and human medicine with no side effect on eukaryotic cells. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2210-9099 |
| Relation: | http://ophrp.org/upload/phrp-10-1/ophrp-10-020.pdf; https://doaj.org/toc/2210-9099 |
| DOI: | 10.24171/j.phrp.2019.10.1.05 |
| URL الوصول: | https://doaj.org/article/78b1b6edb5024d94a4634f4221d7811c |
| رقم الأكسشن: | edsdoj.78b1b6edb5024d94a4634f4221d7811c |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 22109099 |
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| DOI: | 10.24171/j.phrp.2019.10.1.05 |