دورية أكاديمية

Analysis of Three Compounds in Flos Farfarae by Capillary Electrophoresis with Large-Volume Sample Stacking

التفاصيل البيبلوغرافية
العنوان: Analysis of Three Compounds in Flos Farfarae by Capillary Electrophoresis with Large-Volume Sample Stacking
المؤلفون: Hai-xia Yu, Zeng-Yan Hao, Lu Li, Ya-yun Huang, Hong-Fen Zhang, An-jia Chen
المصدر: International Journal of Analytical Chemistry, Vol 2017 (2017)
بيانات النشر: Hindawi Limited, 2017.
سنة النشر: 2017
المجموعة: LCC:Analytical chemistry
مصطلحات موضوعية: Analytical chemistry, QD71-142
الوصف: The aim of this study was to develop a method combining an online concentration and high-efficiency capillary electrophoresis separation to analyze and detect three compounds (rutin, hyperoside, and chlorogenic acid) in Flos Farfarae. In order to get good resolution and enrichment, several parameters such as the choice of running buffer, pH and concentration of the running buffer, organic modifier, temperature, and separation voltage were all investigated. The optimized conditions were obtained as follows: the buffer of 40 mM NaH2P04-40 mM Borax-30% v/v methanol (pH 9.0); the sample hydrodynamic injection of up to 4 s at 0.5 psi; 20 kV applied voltage. The diode-array detector was used, and the detection wavelength was 364 nm. Based on peak area, higher levels of selective and sensitive improvements in analysis were observed and about 14-, 26-, and 5-fold enrichment of rutin, hyperoside, and chlorogenic acid were achieved, respectively. This method was successfully applied to determine the three compounds in Flos Farfarae. The linear curve of peak response versus concentration was from 20 to 400 µg/ml, 16.5 to 330 µg/mL, and 25 to 500 µg/mL, respectively. The regression coefficients were 0.9998, 0.9999, and 0.9991, respectively.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1687-8760
1687-8779
Relation: https://doaj.org/toc/1687-8760; https://doaj.org/toc/1687-8779
DOI: 10.1155/2017/3813879
URL الوصول: https://doaj.org/article/78b8e8c2c99041af9e712c4e0e6b17b2
رقم الأكسشن: edsdoj.78b8e8c2c99041af9e712c4e0e6b17b2
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:16878760
16878779
DOI:10.1155/2017/3813879