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Cloning, Expression, and Characterization of Thermotolerant Manganese Superoxide Dismutase from Bacillus sp. MHS47

التفاصيل البيبلوغرافية
العنوان: Cloning, Expression, and Characterization of Thermotolerant Manganese Superoxide Dismutase from Bacillus sp. MHS47
المؤلفون: Supatra Areekit, Paisarn Khawsak, Arda Pakpitchareon, Kajeenart Potivejkul, Gaysorn Chansiri, Kosum Chansiri, Pornpimon Kanjanavas
المصدر: International Journal of Molecular Sciences, Vol 12, Iss 1, Pp 844-856 (2011)
بيانات النشر: MDPI AG, 2011.
سنة النشر: 2011
المجموعة: LCC:Biology (General)
LCC:Chemistry
مصطلحات موضوعية: thermotolerant, MnSOD, cloning, expression, Bacillus sp., Biology (General), QH301-705.5, Chemistry, QD1-999
الوصف: A superoxide dismutase gene from thermotolerant Bacillus sp. MHS47 (MnSOD47) was cloned, sequenced, and expressed. The gene has an open reading frame of 612 bp, corresponding to 203 deduced amino acids, with high homology to the amino acid sequences of B. thuringiensis (accession no. EEN01322), B. anthracis (accession no. NP_846724), B. cereus (accession no. ZP_04187911), B. weihenstephanensis (accession no. YP_001646918), and B. pseudomycoides. The conserved manganese-binding sites (H28, H83, D165, and H169) show that MnSOD47 has the specific characteristics of the manganese superoxide dismutase (MnSOD) enzymes. MnSOD47 expressed an enzyme with a molecular weight of approximately 22.65 kDa and a specific activity of 3537.75 U/mg. The enzyme is active in the pH range 7–8.5, with an optimum pH of 7.5, and at temperatures in the range 30–45 °C, with an optimum temperature of 37 °C. Tests of inhibitors and metal ions indicated that the enzyme activity is inhibited by sodium azide, but not by hydrogen peroxide or potassium cyanide. These data should benefit future studies of MnSODs in other microorganisms and the biotechnological production of MnSOD47, and could also be used to develop a biosensor for the detection of antioxidants and free radical activity. In the future, this basic knowledge could be applicable to the detection of cancer risks in humans and therapeutic treatments.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1422-0067
Relation: http://www.mdpi.com/1422-0067/12/1/844/; https://doaj.org/toc/1422-0067
DOI: 10.3390/ijms12010844
URL الوصول: https://doaj.org/article/c78f91804ade437d8800f03d4faf61c3
رقم الأكسشن: edsdoj.78f91804ade437d8800f03d4faf61c3
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:14220067
DOI:10.3390/ijms12010844